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Journal of Genetic Resources، جلد ۶، شماره ۱، صفحات ۷۷-۸۴
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
MiR-490-5p Functions as an OncomiR in Breast Cancer by Targeting NFATc4 |
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| چکیده انگلیسی مقاله |
Breast cancer is a serious health problem worldwide in women. MicroRNAs are small non-coding RNAs of 18–25 nucleotides in length that post-transcriptionally modulate gene expression. MiR-490 has been reported as a tumor suppressor and oncomiR microRNA in breast cancer with two separate targets, NFAT and Rho. NFAT is one of the targets for miR-490 but the relationship between hsa-miR-490 and NFATc3, NFATc4 are not clear yet. Except for NFAT5, the other members of NFAT are activated by Ca2+ influx in the cell, either via the PLC-γ pathway or via store-operated Ca2+ entry, typically in T lymphoid cells. In a cross-sectional comparative study, peripheral blood samples were collected from 30 subjects with breast cancer and 30 healthy individuals as a control group. Gene expression analysis of peripheral blood mononuclear cells (PBMCs) was performed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to study the NFATc3, NFATc4, and hsa-miR-490-5p gene expression alterations. As per the obtained results, a significant decrease was observed in the expression level of NFATc4 (P< 0.05), while hsa-miR-490-5p expression found to be elevated in PBMCs of breast cancer patient (P< 0.05). Expression changes were not significant for NFATc3 gene (P>0.05). Taken together findings of this study indicated that serum hsa-miR-490-5p acts as an oncomiR by direct targeting the NFATc4. |
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| کلیدواژه‌های انگلیسی مقاله |
hsa-miR-490-5p, NFATc3, NFATc4, Breast cancer, PBMCs |
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| نویسندگان مقاله |
Amirhassan Nikfarjam |
Department of Laboratory Sciences, Kashan Branch, Islamic Azad University, Kashan, Iran
Mohsen Gholami |
Department of Laboratory Sciences, Kashan Branch, Islamic Azad University, Kashan, Iran
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| نشانی اینترنتی |
http://sc.journals.umz.ac.ir/article_2589_6e37784d224d76e1fed90dfd98b97c0a.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2082/article-2082-2415408.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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