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Iranian Biomedical Journal، جلد ۲۴، شماره ۴، صفحات ۲۴۳-۲۵۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Carvacrol and Thymol Attenuate Cytotoxicity Induced by Amyloid β25-35 Via Activating Protein Kinase C and Inhibiting Oxidative Stress in PC12 Cells |
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| چکیده انگلیسی مقاله |
Background: Our previous findings indicated that carvacrol and thymol alleviate cognitive impairments caused by Aβ in rodent models of AD. In this study, the neuroprotective effects of carvacrol and thymol against Aβ25-35-induced cytotoxicity were evaluated, and the potential mechanisms were determined. Methods: PC12 cells were pretreated with Aβ25-35 for 2 h, followed by incubation with carvacrol or thymol for additional 48 h. Cell viability was measured by MTT method. A flurospectrophotometer was employed to observe the intracellular ROS production. PKC activity was analyzed using ELISA. Results: Our results indicated that carvacrol and thymol could significantly protect PC12 cells against Aβ25-35-induced cytotoxicity. Furthermore, Aβ25-35 could induce intracellular ROS production, while carvacrol and thymol could reverse this effect. Moreover, our findings showed that carvacrol and thymol elevate PKC activity similar to Bryostatin-1, as a PKC activator. Conclusion: This study provided the evidence regarding the protective effects of carvacrol and thymol against Aβ25–35-induced cytotoxicity in PC12 cells. The results suggested that the neuroprotective effects of these compounds against Aβ25-35 might be through attenuating oxidative damage and increasing the activity of PKC as a memory-related protein. Thus, carvacrol and thymol were found to have therapeutic potential in preventing or modulating AD. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
| Zahra Azizi
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
| Mona Salimi
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
| Amir Amanzadeh
Department of Cell Bank, Pasteur Institute of Iran, Tehran, Iran
| Nahid Majelssi
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
| Nasser Naghdi
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
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| نشانی اینترنتی |
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-812&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-2380499.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Related Fields |
| نوع مقاله منتشر شده |
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