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Iranian Journal of Microbiology، جلد ۱۲، شماره ۱، صفحات ۳۷-۴۲
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عنوان انگلیسی A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain
چکیده انگلیسی مقاله Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis. Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs. Results: TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay. Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.
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نویسندگان مقاله | Mohammad Sekhavati
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Ashraf Mohabati Mobarez
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Seyed Davar Siadat
Microbiology Research Center (MRC) Pasteur, Institute of Iran, Tehran, Iran


| Mojtaba Noofeli
Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/2267
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