Iranian Journal of Basic Medical Sciences، جلد ۲۳، شماره ۴، صفحات ۵۳۴-۵۴۳
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عنوان انگلیسی Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro
چکیده انگلیسی مقاله Objective(s): Malaria is an important parasitic disease with high morbidity and mortality in tropical areas. Resistance to most antimalarial drugs has encouraged the development of new drugs including natural products. Venom is a complex mixture of active pharmaceutical ingredients. The purpose of this study was to investigate the antimalarial activity of purified fractions of Naja naja oxiana. Materials and Methods: Lyophilized venom was purified with a Sephacryl S-200 HR column and the fractions lyophilized and inhibitory concentration 50% against Plasmodium falciparum 3D7 in vitro obtained. The 4th fraction was run on a Mono Q column, and activity against P. falciparum was detected by lactate dehydrogenase assay and purity by SDS PAGE. Large scale culture of the parasite was carried out with and without the active fraction on the ring stage for 48 hr. The parasites were collected and lyophilized and analyzed by 1HNMR. Chemometrics studies were performed using MATLAB, differentiating metabolites were identified by Human Metabolic Database, and metabolic pathways by the Metaboanalyst online package. Results: The active fraction from the ion exchange column had a 50% inhibitory concentration of 0.026 µg/ml on P. falciparum in vitro (P
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نویسندگان مقاله | Fatemeh Hajialaiani
Medical Parasitology Department, School of Medicine -International Campus, Iran University of Medical Sciences, Tehran, Iran


| Taher Elmi
Parasitology and Mycology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Maryam Mohammadi
Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran


| Delavr Shahbazzadeh
Laboratory of Venom and Bio Therapeutics Molecules, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Fatemeh Ghafarifar
Department of Parasitology, Faculty of Medical Sciences, Tarbiat modares University Tehran, Iran


| Abolhossein Dalimi Asl
Department of Parasitology, Faculty of Medical Sciences, Tarbiat modares University Tehran, Iran


| Mohammad Arjmand
Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran


| Fatemeh Tabatabaie
Parasitology and Mycology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Zahra Zamani
Biochemistry Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran. Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_14975.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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