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Journal of Kerman University of Medical Sciences، جلد ۲۶، شماره ۵، صفحات ۳۳۹-۳۴۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
High Prevalence of CTXM-15 Type Extended-Spectrum Beta-Lactamase Among Clinical Isolates of Klebsiella Pneumoniae |
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| چکیده انگلیسی مقاله |
Background: Production of β–lactamases by enterobacteriacea, especially Klebsiella pneumoniae, is one of the emerging health problems in the world. The purpose of this study was to assess the frequency of blaCTX-M15 gene in K. pneumoniae isolates and determine the molecular diversity of CTXM producing isolates. Methods: In this descriptive cross-sectional study, 100 K. pneumoniae strains were tested for susceptibility to cephalosporins category by using disk diffusion method and the prevalence of blaCTX-M-1, blaCTX-M-2, blaCTX-M-9 and blaCTX-M-15 genesin these isolates were determined by PCR method. Eventually, a number of isolates were sequenced and typed using single locus sequence typing (SLST) of blaCTX-M group1 gene. The phylogenetic relatedness of all CTX-M producing isolates was determined using enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). Results:The rates of resistance to cephalosporins category were in the following order: cefepime(57%), ceftazidime (54%), cefotaxime (36%), cefoxitine (32%), and ceftriaxone (31%). Of all 100 K. pneumoniae isolates, 31 were harboring CTX-M genes, and the blaCTX-M-15 (77.5%) was the most common of the genes investigated. The results of SLST showed that all of the sequenced isolates were divided into two groups. ERIC-PCR method represented ten different genotypes in CTX-M positive isolates. Conclusion: The results of this study showed that one of the most important reasons for resistance to cephalosporins is the presence of blaCTX-M genes. In addition, there was a high genetic variation among blaCTX-M genes positive isolates. |
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| نویسندگان مقاله |
Leila Ahmadkhan Beygi |
MSC of microbiology, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
Maryam Ghane |
Assistant Professor, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
Laleh Babaeekhou |
Assistant Professor, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
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| نشانی اینترنتی |
http://jkmu.kmu.ac.ir/article_89541_44d3c5f466ebabeaa06aa338ab05a3be.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/93/article-93-2053052.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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