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Avicenna Journal of Medical Biotechnology، جلد ۹، شماره ۴، صفحات ۱۷۶-۱۸۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Lithium Chloride can Induce Differentiation of Human Immortalized RenVm Cells into Dopaminergic Neurons |
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| چکیده انگلیسی مقاله |
Background: Stem cell-based therapy is a novel strategy for the treatment of neuro-degenerative diseases. The transplantation of fully differentiated cells instead of stem cells in order to decrease serious adverse complications of stem cell therapy is a new idea. In this study, the effect of lithium chloride on dopaminergic differentiation of human immortalized RenVm cells was investigated in order to access a population of fully differentiated cells for transplantation in Parkinson disease. Methods: The immortalized RenVm cells were induced to dopaminergic differentiation using a neurobasal medium supplemented with N2 and different concentrations (1, 3, 6 mM) of Lithium Chloride (LiCl) for 4, 8 and 12 days. The efficiency of dopaminergic differentiation was evaluated using immunocytochemistry and western blot techniques for tyrosine hydroxylase and β-catenin marker expression. Results: Our results indicated that LiCl can promote dopaminergic differentiation of RenVm cells in a dose-dependent manner. Conclusion: It can be concluded that LiCl is able to facilitate dopaminergic differenti-ation of cultured cells by affecting Wnt-frizzled signaling pathway. |
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| کلیدواژه‌های انگلیسی مقاله |
Beta catenin, Cell differentiation, Lithium, Wnt proteins |
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| نویسندگان مقاله |
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| نشانی اینترنتی |
http://www.ajmb.org/En/Article.aspx?id=287 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/133/article-133-1975090.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
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