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International Journal of Hematology-Oncology and Stem Cell Research، جلد ۱۲، شماره ۳، صفحات ۲۱۹-۲۲۸
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عنوان انگلیسی Differentiation of Wharton's Jelly Derived Mesenchymal Stem Cells into Insulin Producing Cells
چکیده انگلیسی مقاله Background: Diabetes caused by insulin production disturbance is considered as the most common metabolic disorder all over the world. Diabetes may outbreak because of low insulin secretion by Islets of Langerhans β-cells, insulin resistance or both of them. In this way, using stem cells, which have the capability to differentiate into Pancreatic β-cells, is one of novel methods in this field. MSCs are the most important candidates for cellular therapy. Materials and Methods: Insulin level was examined using ELIZA method. In order to examine the morphology of differentiated cells, they were stained by Dithizone. Insulin-producer cells are cells which turn into red as a result of staining. Specific gene involving insulin-producing cells was evaluated by Real Time-PCR method. Results: The ELISA results showed that the treated cells secreted more insulin than the control group. Moreover, we found differentiation of MSCs toward insulin-secreting cells. In order to evaluate insulin production in clusters on day 21 of differentiation, we used dithizone (DTZ) staining. PDX-1 gene was confirmed by RT- PCR analysis. Conclusion: In this study, we differentiated MSCs into insulin-producing cells in vitro. It is concluded that MSCs may be considered as an excellent candidate in β-cell therapy in diabetes patients.
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نویسندگان مقاله | Hossein Ranjbaran
Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Saeid abediankenari
Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Alireza Khalilian
Department of Biostatistics and Community Medicine, School of Medicine, Mazandaran University of Medical Sciences, Sari


| Zahra Rahmani
Department of Obstetrics, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Marzei Momeninezhad Amiri
School of Traditional Medicine, Iran University of Medical Sciences, Tehran, Iran


| Zahra Hosseini khah Hosseini Khah
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/828
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