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International Journal of Radiation Research، جلد ۴، شماره ۴، صفحات ۱۸۷-۱۹۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Production and evaluation of [67Ga]-DTPA-Rituximab |
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| چکیده انگلیسی مقاله |
Background: In order to obtain an anti-CD20 conjugate to be used in future therapeutic studies with therapeutic radioisotopes, 67Ga-labeled antibody was prepared as a model of metal chelated immunoconjugate for preliminary dosimetric and biodistribution studies. Materials and Methods: Rituximab was labeled with [67Ga]-gallium chloride after residulation with freshly prepared cyclic DTPAdianhydride. The best results of the conjugation were obtained by the addition of 1 ml of a rituximab pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=8) to a glass tube pre-coated with DTPAdianhydride (0.01 mg) at 25|o|C with continuous mild stirring for 30 min. The final isotonic 67Ga-DTPArituximab complex was checked by gel electrophoresis for radiolysis/chemolysis control. Radio-TLC was performed to ensure the formation of only one species. Preliminary in vivo studies in normal rat model were performed to determine the biodistribution of the radioimmunoconjugate up to 6 hours. Results: Radio-thin layer chromatography showed an overall radiochemical purity of 96-99% at optimized conditions (specific activity =300-500 MBq/mg, labeling efficiency 77%). Gel electrophoresis showed no protein cleavage after radiolabeling. Conclusion: Preliminary in vivo studies in normal rat model showed [67Ga]-DTPA-rituximab is a good probe for bio-dosimetry of therapeutic rituximab conjugates. Iran. J. Radiat. Res., 2007 4 (4): 187-193 |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
علیرضا جلیلیان | a r jalilian
department of cyclotron amp;amp; nuclear medicine, nuclear research center for agriculture and medicine, karaj, iran
l mirsadegh | l. mirsadegh
department of biochemistry, payam-e-noor university, tehran, iran
r حاجی حسینی | r haji hosseini
department of biochemistry, payam-e-noor university, tehran, iran
s رجبی فر | s. rajabifar
department of cyclotron amp;amp; nuclear medicine, nuclear research center for agriculture and medicine, karaj, iran
f bolurinovin | f. bolurinovin
department of cyclotron amp;amp; nuclear medicine, nuclear research center for agriculture and medicine, karaj, iran
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| نشانی اینترنتی |
http://www.ijrr.com/browse.php?a_code=A-10-1-222&slc_lang=en&sid=en |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Radiation Biology |
| نوع مقاله منتشر شده |
تحقیق بدیع |
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