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Research in Pharmaceutical Sciences، جلد ۱۴، شماره ۲، صفحات ۱۶۷-۱۷۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro
چکیده انگلیسی مقاله Herpes simplex virus type 1 (HSV-1) infections are one of the most common diseases in human population. HSV-1 causes subclinical, mild to severe diseases, especially in immunocompromised patients. Acyclovir has been used to reduce manifestations of HSV-1 infections. The extensive use of this drug has led to the development of resistant strains. Thus, designing a novel anti-herpes drug with different mechanisms of action is urgently needed. Cellular microRNAs (miRNAs) have direct antiviral effects in addition to their regulatory functions. In this study we used a novel miRNA (hsa-miR-7704), expressed in macrophages, to inhibit HSV-1 lytic infection in HeLa cells. Synthesized hsa-miR-7704 mimics were transfected into HSV-1 infected HeLa cell. The inhibitory effects of the miRNA were evaluated by plaque assay, real time polymerase chain reaction and the viral titers were measured by the 50% tissue culture infective dose (TCID 50 ). The viral titer and cell cytopathic effect were dramatically decreased in HeLa cells transfected with hsa-miR-7704 (50 and 100 nM), compared with HSV-1 infected cells alone or transfected with the mock miRNA control. These results suggest that hsa-miR-7704 inhibits HSV-1 replication efficiently in vitro . This may provide an alternative mechanism to prevent HSV-1 infections.
کلیدواژه‌های انگلیسی مقاله Antiviral therapy,HSV-1,Micro RNA,miR-has-7704,miR-SX1.
نویسندگان مقاله | Mehdi Shabani
1Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Bahram Nasr Esfahani
2Department of Microbiology, School of Medicine, Kashan University of Medical Sciences, Kashan, I.R. Iran.


| Bahar Sadegh Ehdaei
1Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Sharareh Moghim
1Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Arezoo Mirzaei
3Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Mohammadreza Sharifi
1Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Leili Mouhebat


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1892
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/115/article-115-1955266.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
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