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International Journal of Radiation Research، جلد ۱۳، شماره ۲، صفحات ۱۵۱-۱۵۶
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عنوان انگلیسی Radio-adaptive response of peripheral blood lymphocytes following bystander effects induced by preirradiated CHO-K1 cells using the micronucleus assay
چکیده انگلیسی مقاله Background: Radio-adaptive response and bystander effects are known phenomena occurring in cells following exposure to ionizing radiation (IR). In this study we examined possible radio-adaptation of lymphocytes following bystander effects induced by CHO-K1 cells. Materials and Methods: Whole blood and CHO-K1 cells were cultured in RPMI-1640 complete medium. Cells were separately irradiated with various doses of gamma rays. A co-culture was set to examine the bystander effects induced by CHO-K1 cells on lymphocytes exposed later to a challenge dose of 4 Gy. Treated cells were exposed to cytochalasin-B to arrest cells in cytokinesis stage. Micronucleus (MN) as end point was scored in binucleate cells after staining in Giemsa. Results: The frequency of MN increased significantly with increasing dose of radiation both in lymphocytes and CHO-K1 cells (p< 0.001). Although, no significant difference was observed between control non-irradiated cells and those exposed to 0.2 Gy (p>0.05). Co-culture of the non-irradiated lymphocytes with pre-irradiated CHO-K1 cells significantly reduced the mean frequency of MN in lymphocytes irradiated with a dose of 4Gy (p< 0.001). Conclusion: Results showed that bystander effects induced by gamma-irradiated CHO-K1 cells led to induction of radio-adaptive response in lymphocytes. The mechanism by which radio-adaptive response is induced following bystander effect is not clearly known, however cellular signaling and genome instability induced in cells indirectly might be considered as possible triggering events for radio-adaptive response.
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نویسندگان مقاله sh شیرانی | sh. shirani
school of paramedicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

h مزدارانی | h. mozdarani
department of medical genetics, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

a محمودزاده | a. mahmoodzadeh
novin medical radiation institute, tehran, iran


m سلیمی | m. salimi
department of medical genetics, medical biotechnology institute, national institute of genetic engineering and biotechnology, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

نشانی اینترنتی http://www.ijrr.com/browse.php?a_code=A-10-1-553&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiation Biology
نوع مقاله منتشر شده تحقیق بدیع
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