International Journal of Radiation Research، جلد ۱۴، شماره ۱، صفحات ۹-۱۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In-vitro evaluation of rutin and rutin hydrate as potential radiation countermeasure agents
چکیده انگلیسی مقاله Background: DNA damage is one of the major consequences of radiation exposure onto the biological systems. A series of compounds including flavanoids were found to render DNA protection against radiation damage. In this study we elucidated the potential of rutin and rutin hydrate to protect plasmid DNA against damage induced by irradiation. Materials and Methods: DPPH and hydroxyl radical scavenging assays were performed to assess the antiradical potential of rutin and rutin hydrate. Absorption measurements were performed to assess binding parameters of rutin and rutin hydrate with calf thymus (CT)-DNA. Plasmid relaxation assay was performed to compare the radio protective potential of rutin and rutin hydrate against gamma irradiation mediated oxidative damage of pET28 plasmid DNA. Results: DPPH· assay indicated fast reaction kinetics for rutin and rutin hydrate. However antiradical parameter in terms of EC50 suggested better scavenging capacity for rutin hydrate as compared to rutin. Hydroxyl radical scavenging assay further suggested that both the compounds displayed significant reduction in hydroxyl radicals. Absorption binding study with CT-DNA suggested that rutin hydrate has better binding constant value (Ka = 8.257 x 104 M-1) compared to Ka = 1.834 x 104 M-1 for rutin. Plasmid relaxation study demonstrated that plasmid DNA remains predominantly in super-coiled form in the presence of both rutin and rutin hydrate after exposure to 100 Gy of g-radiation. Conclusion: The mechanistic studies suggested that binding and scavenging capacity of rutin hydrate and rutin contributes towards DNA radioprotection. This study may be helpful in devising potent radioprotector molecules helpful for the radiotherapy treatment.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | h. ojha
department of radiation genetics and epigenetics, institute of nuclear medicine and allied sciences, delhi 110054, india


k شارما | k. sharma
department of radiation genetics and epigenetics, institute of nuclear medicine and allied sciences, delhi 110054, india


s kallepalli | s. kallepalli
department of radiation genetics and epigenetics, institute of nuclear medicine and allied sciences, delhi 110054, india


s raina | s. raina
department of radiation genetics and epigenetics, institute of nuclear medicine and allied sciences, delhi 110054, india


p k agrawala | p k agrawala
department of radiation genetics and epigenetics, institute of nuclear medicine and allied sciences, delhi 110054, india
نشانی اینترنتی http://www.ijrr.com/browse.php?a_code=A-10-1-591&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiation Biology
نوع مقاله منتشر شده تحقیق بدیع
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات