این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Kerman University of Medical Sciences، جلد ۲۶، شماره ۲، صفحات ۹۵-۱۰۹
عنوان فارسی Cardiogel به عنوان یک میکروکنواکتیو آموزشی برای تشخیص درون سلولی سلولهای بنیادی مزانشیمی استخوان مغز استخوان به قلب و عروق
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Cardiogel as an Instructive Microenvironment for in vitro Differentiation of Bone Marrow- Derived Mesenchymal Stem Cells into Cardiomyocytes
چکیده انگلیسی مقاله Background: Stem cell therapy has been developed as an effective treatment method for the heart failure. Also, extracellular matrix has shown the positive effects in stem cell differentiation and myocardial tissue organization. Cardiogel is a native cardiac extracellular matrix (ECM) derived from cardiac fibroblasts. In the present study the role of cardiogel is examined for its cardiomyogenic potential on mouse bone marrow- derived mesenchymal stem cells (BM-MSCs). Method: The BM-MSCs were isolated from six-week-old mice. Cardiac fibroblasts were collected from neonatal heart mice and the cells were seeded on 0.2% gelatin pre-coated plates for up to 21 days. Then, the decellularization was performed via enzymatic digestion. For cardiomyocyte differentiation, the BM-MSCs were plated on matrix-coated plates (Cardiogel, CCP), Matrigel-coated plates (MCP) and gelatin-coated plates (GCP) as a control group at a density of 1 × 104 cells per cm2 in CM containing 3 mM 5-azacytidine. Results: The results proved that cardiogel is capable to promote the cardiomyogenic differentiation of BM-MSCs. Cardiogel had a great influence on cellular adhesion, gene expression and cardiomyocyte differentiation compared to MCP and GCP. Gene expression analysis showed that cardiac specific markers were expressed in BM-MSCs has grown in CCP that represented the cardiac-specific differentiation. Conclusion: Our results suggest that cardiogel is an effective ECM that can improve the cardiomyogenic differentiation of BM-MSCs, and it can be used as a possible therapeutic approach in cardiac tissue regeneration.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله Maryam Nazm Bojnordi |
Assistant Professor, Immunogenetic Research Center & Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Nourollah Rezaei |
Assistant Professor, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Zahra Bagheri-Hosseinabadi |
Assistant Professor, Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran & Institute of Neuropharmacology, Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran

Sina Mojaverrostami |
Ph.D. Student of Anatomy, Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Hatef Ghasemi Hamidabadi |
Associate Professor, Department of Anatomy & Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Mehrdad Naghikhani |
Assistant Professor, Department of Basic Sciences, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran

Mostafa Latifpour |
Assistant Professor of Anatomy, Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

نشانی اینترنتی http://jkmu.kmu.ac.ir/article_89193_5f596ba02fdc0c74e97464a1f9b2abe2.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/93/article-93-1617749.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات