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Journal of Agricultural Science and Technology، جلد ۱۸، شماره ۵، صفحات ۱۴۱۹-۱۴۲۹
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عنوان انگلیسی Genetic Variability, Structure Analysis, and Association Mapping of Resistance to Broomrape (Orobanche aegyptiaca Pers.) in Tobacco
چکیده انگلیسی مقاله Broomrape is a debilitating holoparasiting weed in tobacco (Nicotiana tabacum L.) fields with devastating effects on its production. In this study, the reaction of 89 tobacco genotypes was evaluated against broomrape (Orobanche aegyptiaca) in randomized complete block design with three replications during two years. In each year, genotypes were planted in both non-inoculated and inoculated conditions where the soil of pots was mixed with 0.06 g of broomrape seed. Considering the average data of two years, studied genotypes did not show infection to broomrape at non-inoculated condition, whereas in inoculated condition, the majority of genotypes showed infection to broomrape. Two genotypes including ‘TB 22’ and ‘Kramograd NHH 659’ did not show any infection to broomrape in inoculated condition. In a molecular experiment, the fingerprint of tobacco genotypes was prepared with 26 SSR loci. Using model-based Bayesian approach, the studied association panel was divided into three subgroups. The D¢ was used to test the LD between pairs of SSR loci using the software package TASSEL. 7.08% of possible SSR locus pairs showed significant level of linkage disequilibrium (P
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نویسندگان مقاله r درویش زاده |
department of plant breeding and biotechnology, faculty of agriculture, urmia university, urmia, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه ارومیه (Urmia university)

نشانی اینترنتی http://jast.modares.ac.ir/article_14966_d3d35162e26e7d31b48c3cc81b488171.pdf
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