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Iranian Journal of Microbiology، جلد ۱۱، شماره ۱، صفحات ۳۹-۴۷
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عنوان انگلیسی Vaccine potential of LenA and LcpA proteins of Leptospira interrogans in combination with Escherichia coli heat-labile enterotoxin, B subunit (LTB)
چکیده انگلیسی مقاله Background and Objectives: Leptospirosis is a zooanthroponosis caused by the genus of Leptospira. It is an emerging public health problem due to its increasing incidence. The achievement to a vaccine that prevent from entrance of Leptospira interrogans to the deeper tissues of the host is needed. This study aimed to investigate the immunogenicity of LcpA (rLcpA) and LenA (rLenA) recombinant proteins in combination with LTB (rLTB) recombinant protein as an adjuvant against leptospiral infection in hamsters. Materials and Methods: The genes encoding these proteins were cloned into pGH cloning vector and then lenA, lcpA and ltb genes subcloned into pET-15b and pET-28a expression vectors, respectively. The hamsters were immunized with the purified recombinant proteins and challenged with Leptospira interrogans for evaluation of their survival. The antibody responses to the recombinant proteins were determined by ELISA. Then, data entered into SPSS software. Statistical Kruskal–Wallis test was used to compare the significant differences among different groups. The groups with significant differences were further analyzed by post hoc tests. The p value < 0.05 statistically was considered significant. Results: Immunized hamsters with rLenA-plus-rLTB, rLcpA-plus-rLTB and rLenA-plus-rLcpA-plus-rLTB proteins showed 60%, 74%, and 80% survival rates, respectively. A significant amount of interleukin-17 (IL-17), interleukin-4 (IL-4) and gamma interferon (IFNγ) cytokines were produced in immunized hamsters. Conclusion: Based on our findings, rLcpA and rLenA proteins in combination with rLTB can protect the hamsters against L. interrogans and effectively induce a protective antibody response. Thus, these proteins can be used as an additional prophylactic tool against leptospira.
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نویسندگان مقاله | Mehran Ghazali-Bina
Department of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Mohammad Reza Pourmand
Department of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Abbas Mirshafiey
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Ronak Bakhtiari
Department of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Azad Khaledi
Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran; Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran


| Hamid Kazemian
Department of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Davoud Afshar
Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran


| Muhammad Ibrahim Getso
Department of Medical Mycology, School of Public Health, Tehran University of Medical Sciences, Intenational College, Tehran, Iran


| Saeid Eshraghi
Department of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/1969
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