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Iranian Journal of Basic Medical Sciences، جلد ۲۲، شماره ۳، صفحات ۳۰۲-۳۰۹
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عنوان انگلیسی Effect of IL-2 co-expressed or co-inoculated with immuno-dominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice
چکیده انگلیسی مقاله Objective(s): The results of studies on vaccine development for foot-and-mouth disease (FMD) virus show that the use of inactivated vaccines for FMD virus is not completely effective. Novel vaccinations based on immuno-dominant epitopes have been shown to induce immune responses. Furthermore, for safety of immunization, access to efficient adjuvants against FMD virus seems to be critical.Materials and Methods: In this study, we produced epitope recombinant vaccines from the VP1 protein of the FMD virus for serotype O of Iran. Constructs were included polytope (tandem-repeat multiple-epitope), polytope coupled with interleukin-2 (polytope-IL 2) as a molecular adjuvant and IL-2. Three expression vectors were constructed and expressed in Escherichia coli BL21 (DE3). To evaluate whether these recombinant vaccines induce immune responses, BALB/c mice were injected with the recombinant vaccines and their immune responses were compared with a negative control group. The humoral and cellular immune responses were measured by ELISA.Results: The results showed that IL-2 co-expressed or co-inoculated with Polytope protein enhances the immune effect of multiple epitope recombinant vaccine against FMD virus. The results of total immunoglobulin G (IgG), IgG1, and IgG2a levels and secretion of interferon gamma (IFN-γ), IL-4 and IL-10 revealed that there were significant differences between negative control group and other injected mice with the recombinant vaccines (P<0.05).Conclusion: Observations indicated that the epitope recombinant plasmid of the VP1 protein co-expressed or co-inoculated with IL-2 was effective in inducing an enhanced immune response. Therefore, IL-2 can be recommended as a potential adjuvant for epitope recombinant vaccine of the VP1 protein from FMD virus.
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نویسندگان مقاله | Mohammad Doosti
Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran


| Mohammadreza Nassiri
Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran|Recombinant Proteins Research Group, Research Institute of Biotechnology and genetic group, Ferdowsi University of Mashhad, Mashhad, Iran


| Khadijeh Nasiri
Department of Exercise Physiology, Faculty of Sport Science, University of Mazandaran, Babolsar, Iran 4 Razi Vaccine and Serum Research Institute, Mashhad, Iran


| Mojtaba Tahmoorespur
Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran


| saeed Zibaee
Razi Vaccine and Serum Research Institute, Mashhad, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_12230.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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