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Reports of Biochemistry and Molecular Biology، جلد ۱، شماره ۲، صفحات ۸۲-۸۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Construction of an Expression Plasmid (Vector) Encoding Brucella melitensis Outer Membrane Protein, a Candidate for DNA Vaccine |
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| چکیده انگلیسی مقاله |
Background: DNA vaccination with plasmid encoding bacterial, viral, and parasitic immunogens has been shown to be an attractive method to induce efficient immune responses. Bacteria of the genus Brucella are facultative intracellular pathogens for which new and efficient vaccines are needed. Methods: To evaluate the use of a DNA immunization strategy for protection against brucellosis, a plasmid containing the DNA encoding the Brucella melitensis (B. melitensis) 31 kDa outer membrane protein, as a potent immunogenic target, was constructed. Results: The constructed plasmid, pcDNA3.1+omp31, was injected intramuscularly into mice and the expression of omp31 RNA was assessed by RT-PCR. The integrity of the pcDNA3.1+omp31 construct was confirmed with restriction analysis and sequencing. Omp31 mRNA expression was verified by RT-PCR. Conclusion: Our results indicate that the pcDNA3.1+omp31 eukaryotic expression vector expresses omp31 mRNA and could be useful as a vaccine candidate. |
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| کلیدواژه‌های انگلیسی مقاله |
Brucella melitensis, DNA Vaccine, Omp31, PcDNA3.1 |
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| نویسندگان مقاله |
فاطمه واحدی | fatemeh vahedi
tel 98 511- 8431780; fax 98 511- 8420430
الناز قربانی | elnaz ghorbani
microbiology department, al-zahra university, tehran, iran
طاهره فلسفی | tahereh falsafi
microbiology department, al-zahra university, tehran, iran
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| نشانی اینترنتی |
http://www.rbmb.net/browse.php?a_code=A-10-1-13&slc_lang=en&sid=en |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
تخصصی |
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