این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Nanomedicine Journal، جلد ۱، شماره ۵، صفحات ۲۹۲-۲۹۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The cellular uptake of antisense oligonucleotid of E6 mRNA into cervical cancer cells by DOPE-modified hydroxyapatite nanoparticles
چکیده انگلیسی مقاله Objective(s): Although several chemical and physical methods for gene delivery have been introduced, their cytotoxicity, non-specific immune responses and the lack of biodegradability remain the main issues. In this study, hydroxyapatite nanoparticles (NPs) and 1,2-dioleoyl-sn-glycero-3-phosphoethanol​amine (DOPE)-modified hydroxyapatite NPs was coated with antisense oligonucleotide of E6 mRNA, and their uptakes into the cervical cancer cell line were evaluated. Materials and Methods: Calcium nitrate and diammonium phosphate were used for the synthesis of the hydroxyapatite nanoparticle. Thus, they were coated with polyethylene glycol (PEG), DOPE and antisense oligonucleotide of E6 mRNA using a cross-linker. Then, hydroxyapatite NPs and DOPE-modified hydroxyapatite NPs were incubated 48 hours with cervical cancer cells and their uptakes were evaluated by fluorescent microscopy. Results: The hydroxyapatite NPs had different shapes and some agglomeration with average size of 100 nm. The results showed DOPE-modified hydroxyapatite NPs had higher uptake than hydroxyapatite NPs (P< 0.05). Conclusions: Hydroxyapatite NPs conjugated with DOPE are a good choice for gene delivery and silencing of viral genes in cervical cancer cells, but their efficacy should be addressed more in future studies.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله نگین صفارزاده | negin saffarzadeh
department of medical genetic, shahid sadoughi university of medical sciences, yazd, iran


سید مهدی کلانتر | seyed mehdi kalantar
department of medical genetic, shahid sadoughi university of medical sciences, yazd, iran |research and clinical centre for infertility, shahid sadoughi university of medical sciences, yazd, iran


علی جبالی | ali jebali
department of medical genetic, shahid sadoughi university of medical sciences, yazd, iran


سید حسین حکمتی مقدم | seyed hossein hekmatimoghaddam
department of laboratory sciences, faculty of paramedicine, shahid sadoughi university of medical sciences, yazd, iran


محمد حسن شیخها | mohammad hassan sheikhha
department of medical genetic, shahid sadoughi university of medical sciences, yazd, iran | research and clinical centre for infertility, shahid sadoughi university of medical sciences, yazd, iran


احسان فراشاهی | ehsan farashahi
department of medical genetic, shahid sadoughi university of medical sciences, yazd, iran | research and clinical centre for infertility, shahid sadoughi university of medical sciences, yazd, iran
نشانی اینترنتی http://nmj.mums.ac.ir/article_3053.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده 1
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات