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Nanomedicine Journal، جلد ۲، شماره ۳، صفحات ۱۷۵-۱۸۶
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عنوان انگلیسی Preparation of protein-loaded PLGA-PVP blend nanoparticles by nanoprecipitation method: entrapment, Initial burst and drug release kinetic studies
چکیده انگلیسی مقاله Objective(s):Despite of wide range applications of polymeric nanoparticles in protein delivery, there are some problems for the field of protein entrapment, initial burst and controlled release profile. Materials and Methods: In this study, we investigated the influence of some changes in PLGA nanoparticles formulation to improve the initial and controlled release profile. Selected parameters were: pluronic F127, polysorbate 80 as surfactant, pH of inner aqueous phase, L/G ratio of PLGA polymer, volume of inner aqueous phase and addition of polyvinylpyrrolidone as an excipient. FITC-HSA was used as a model hydrophilic drug. The nanoparticles were prepared by nanoprecipitation. Results: Initial release of FITC-HSA from PLGA-tween 80 nanoparticles (opt-4, 61%) was faster than control (PLGA-pluronic) after 2.30 h of incubation. Results showed that decrease in pH of inner aqueous phase to pI of protein can decrease IBR but the release profile of protein is the same as control. Release profile with three phases including a) initial burst b) plateau and c) final release phase was observed when we changed volume of inner aqueous phase and L/G ratio in formulation. Co-entrapment of HSA with PVP and pluronic reduced the IBR and controlled release profile in opt-19. Encapsulation efficiency was more than 97% and nanoparticles size and zeta potentials were mono-modal and -18.99 mV, respectively. Conclusion: In this research, we optimized a process for preparation of PLGA-PVP-pluronic nanoparticles of diameter less than 300 nm using nanoprecipitation method. This formulation showed a decreased initial burst and long lasting controlled release profile for FITC-HSA as a model drug for proteins.
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نویسندگان مقاله شهریار شاکری | shahryar shakeri
department of biotechnology, institute of science and high technology and environmental sciences, graduate university of advanced technology, kerman, iran

سازمان اصلی تایید شده: دانشگاه تحصیلات تکمیلی صنعتی کرمان (Graduate university of advanced technology)

رسول روغنیان | rasoul roghanian
department of biology, faculty of sciences, university of isfahan, isfahan, iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

گیتی امتیازی | giti emtiazi
department of biology, faculty of sciences, university of isfahan, isfahan, iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

cesare errico | cesare errico
department of chemistry and industrial chemistry, university of pisa, via risorgimento 35, 56126 pisa, italy


emo chiellini | emo chiellini
department of chemistry and industrial chemistry, university of pisa, via risorgimento 35, 56126 pisa, italy


federica chiellini | federica chiellini
department of chemistry and industrial chemistry, university of pisa, via risorgimento 35, 56126 pisa, italy
نشانی اینترنتی http://nmj.mums.ac.ir/article_4473.html
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