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Veterinary Research Forum، جلد ۹، شماره ۴، صفحات ۳۲۳-۳۲۸
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عنوان انگلیسی Antimicrobial activity of an antimicrobial peptide against amastigote forms of Leishmania major
چکیده انگلیسی مقاله Zoonotic cutaneous leishmaniasis caused by Leishmania major is a most common type of vector-borne disease in Iran. The pentavalent antimonial drugs have been used in the treatment of cutaneous leishmaniasis for a long time, but drug resistance and some of serious side effects have been reported. Thus, discovery and development of new therapeutic candidates are needed. The CM11 peptide is one of these peptides that its anti-bacterial activity has been proven. This peptide is a short cecropin–melittin hybrid peptide obtained through a sequence combination approach. The aim of this study was to evaluate in vitro anti-leishmanial activity of CM11 peptide against amastigote forms of Leishmania major. In this study, amastigote forms of Iranian strain of L. major (MRHO/IR/75/ER) were cultured in the presence of different concentrations of meglumine antimoniate (Glucantime®) to find the most appropriate in vitro concentration of Glucantime® against L. major amastigotes. Then, the anti-leishmanial activities of various concentrations of CM11 peptide (8, 16, 32 and 64 µM) were evaluated for 24, 48 and 72 hr by DAPI staining. In addition, MTT assay was used to determine the cytotoxic effects of CM11 peptide on murine fibroblast cell line. The results showed that CM11 peptide has antimicrobial activity against Iranian isolate of L. major in the laboratory conditions. It seems that the CM11 peptide has significant potential to be used as a new anti-leishmanial agent.
کلیدواژه‌های انگلیسی مقاله Amastigote, CM11 Peptide, Leishmania major
نویسندگان مقاله Sara khalili |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Mehdi Mohebali |
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Elaheh Ebrahimzadeh |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Parviz Shayan |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Samira Mohammadi-Yeganeh |
Department of Biotechnology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mehrdad Moosazadeh Moghaddam |
Applied Biotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran

Samira Elikaee |
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Behnaz Akhoundi |
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Mohammad Kazem Sharifi-Yazdi |
Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran

نشانی اینترنتی http://vrf.iranjournals.ir/article_33107_a097ed987dedf00f3e6bd9d7897a705a.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/544/article-544-1112777.pdf
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زبان مقاله منتشر شده en
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