این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
International Journal of Molecular and Cellular Medicine، جلد ۱، شماره ۲، صفحات ۱۱۳-۱۱۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Analysis of c.3369+213TA[7-56] and D7S523 microsatellites linked to Cystic Fibrosis Transmembrane Regulator.
چکیده انگلیسی مقاله Cystic fibrosis (CF) is a life-limiting autosomal recessive disorder affecting principally respiratory and digestive system . It is caused by cystic fibrosis transmembrane conductance regulator (CFTR) gene mutation. The aim of this study was to determine the extent of repeat numbers and the degree of heterozygosity for c.3499+200TA(7_56) and D7S523 located in intron 17b and 1 cM proximal to the CFTR gene respectively. Both microsatellites were analyzed by direct electrophoresis of PCR product on 20% polyacrylamide gel in 40 Normal subjects and 40 CF patients originating from North Iran. 9 different alleles were found for D7S523 ranging from 16 to 24 repeats alleles. (CA)20 was the most prevalent allele both in normal individuals and CF patients with 21.3% and 20% frequencies respectively. Heterozygosity frequency of D7S523 in normal individuals and CF patients was 97.5% and 90% respectively. Eighteen different alleles were found for c.3499+200TA(7_56) ranging from 8 to 38 repeats alleles. (TA)9 was the most prevalent allele both in normal individuals and CF patients with 30% and 23.5% frequencies respectively. All normal subjects and 97.5% of CF patients showed heterozyous genotype. The high heterozygosity of the two studied microsatellites witnesses the dynamism of such markers. High degree of heterozygosity of c.3499+200TA(7_56) and D7S523 make these markers, a very useful tool for prenatal diagnosis especially in Iranian population.
کلیدواژه‌های انگلیسی مقاله Cystic Fibrosis, c.3369+213TA[7-56], D7S523, Iran
نویسندگان مقاله وحید خلقی | vahid kholghi oskooei
cellular and molecular biology research center cmbrc , babol university of medical sciences, babol, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)

محمدرضا اسماعیلی دوکی | mohammad reza esmaeili dooki
non-communicable pediatric diseases research center, babol university of medical sciences, babol, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)

هاله اخوان نیاکی | haleh akhavan niaki
cellular and molecular biology research center cmbrc , babol university of medical sciences, babol, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)

نشانی اینترنتی http://www.ijmcmed.org/browse.php?a_code=A-10-24-1&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده 1
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات