Iranian Journal of Basic Medical Sciences، جلد ۲۳، شماره ۳، صفحات ۳۷۶-۳۸۲
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Investigating the inhibitory effect of miR-34a, miR-449a, miR-1827, and miR-106b on target genes including NOTCH1, c-Myc, and CCND1 in human T cell acute lymphoblastic leukemia clinical samples and cell line
چکیده انگلیسی مقاله Objective(s): microRNAs are small non-coding molecules that regulate gene expression in various biological processes. T-cell acute lymphoblastic leukemia (T-ALL) is a malignancy accompanied with genetic aberrations and accounts for 20% of children’s and adult’s ALL. Notch signaling pathway dysregulation occurs in 60% of T-ALL cases. In the present study, we aimed to determine the relationship between miRNAs and genes involved in Notch signaling pathway. Materials and Methods: Considering the role of the pathway and its down-stream genes in proliferation, differentiation, cell cycle, and apoptosis, NOTCH1, c-Myc, and CCND1 genes were selected as target genes. Using bioinformatics studies, miR-34a, miR-449a, miR-1827, and miR-106b were selected as miRNAs targeting the above-mentioned genes. We evaluated these genes and miRNAs in T-ALL clinical samples as well as Jurkat cell line, in which NOTCH1 is overexpressed. Results: Quantitative Real-Time PCR indicated that NOTCH1, c-Myc, and CCND1 were overexpressed in samples with decreased expression of miR-34a. In addition, we observed that samples with decreased expression of miR-449a showed increased expression of NOTCH1 and CCND1. Furthermore, we analyzed the expression of miR-1827 and miR-106b, which target c-Myc and CCND1, respectively. We found out that the expression of miR-1827, miR-106b, and their respective target genes were inversely correlated in 80% and 75% of the cases (r=0.8), respectively. Furthermore, in Jurkat cell line, the expression of target genes was increased while the candidate miRNAs except miR-34a were decreased. Conclusion: These miRNAs can be proposed as biomarkers and new therapeutic targets in T-ALL patients who have NOTCH1 overexpression.
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نویسندگان مقاله | Tohid Naderi
Department of laboratory hematology and blood bank, School of allied medicine, Shahid Beheshti University of medical sciences, Tehran, Iran


| Samira Mohammadi Yeganeh
Medical nanotechnology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran|Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Neda Mohammadi-Hezaveh
Department of laboratory hematology and blood bank, School of allied medicine, Shahid Beheshti University of medical sciences, Tehran, Iran


| Razie Hadavi
Department of Biochemistry, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran|Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Ahmad Gharehbaghian
Department of laboratory hematology and blood bank, School of allied medicine, Shahid Beheshti University of medical sciences, Tehran, Iran


| Nader Vazifeh Shiran
Department of laboratory hematology and blood bank, School of allied medicine, Shahid Beheshti University of medical sciences, Tehran, Iran


| Vahid Fallah Azad
Mahak charity hospital, Tehran, Iran


| Mahdi Paryan
Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_14413.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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