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Iranian Journal of Parasitology، جلد ۱۳، شماره ۲، صفحات ۱۸۶-۱۹۲
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عنوان انگلیسی Comparison of p27 Gene Expression of Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER) by Real-time RT-PCR
چکیده انگلیسی مقاله Background: Cutaneous leishmaniasis (CL) is one of the world health problems. Leishmania major is the etiological agent of zoonotic cutaneous leishmaniasis (ZCL). Promastigote and amastigote are two morphological forms of Leishmania parasites that express different proteins and p27 is an important gene encoding cytochrome c oxidase (COX) component. P27 gene expresses a 27 kDa protein that essential in ATP synthesis. This study aimed to compare p27 gene expression in promastigote and amastigote forms in Iranian strain of L. major (MRHO/IR/75/ER). Methods: This study was conducted in 2015. Clinical isolates of CL patients from north, center, west and south parts of Iran were collected and identified by PCR-RFLP. After RNA extraction of promastigotes and amastigotes and cDNA synthesis, the expression level of p27 gene was compared by real-time RT-PCR. Results: By comparison of expression level between amastigote and promastigote forms of Iranian strain of L. major , up-regulation of p27 gene (2.73 fold) was observed in amastigotes. Moreover, there was no significant difference in p27 gene expression between L. major isolates. Conclusion: p27 gene and protein can be considered as a target in recombinant vaccine production and treatment process.
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نویسندگان مقاله | Samira ELIKAEE
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Center for Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran


| Mehdi MOHEBALI
Dept. of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Hamid ESLAMI
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Sassan REZAEI
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Hamid Reza NAJAFIAN
Dept. of Parasitology, Pasteur Institute of Iran, Tehran, Iran


| Elham KAZEMI-RAD
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Hossein KESHAVARZ
Dept. of Biostatistics and Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Mohammad Reza ESHRAGHIAN
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Homa HAJJARAN
Dept. of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Mohammad Ali OSHAGHI
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Sara AYAZIAN MAVI


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/2201
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-671374.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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