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Iranian Biomedical Journal، جلد ۲، شماره ۱، صفحات ۲۱-۲۵
عنوان فارسی PCR-mediated Expression of the Human GM-CSF Gene in Escherichia coli
چکیده فارسی مقاله Four exons of the human genomic GM-CSF gene were assembled together using gene splicing by overlap extension (SOE) method. The resulting nucleotide sequence was cloned in the pET23a(+) ex‌pression vector under the control of strong bacteriophage T7 transcription and translation signals. The construct obtained was Transferred into the E. coli strain, BL21(DE3) pLysS and IPTG was used for induction of GM-CSF gene and production of the target protein. one mg of protein per liter of cell culture, was obtained as revealed by ELISA.
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عنوان انگلیسی PCR-mediated Expression of the Human GM-CSF Gene in Escherichia coli
چکیده انگلیسی مقاله Four exons of the human genomic GM-CSF gene were assembled together using gene splicing by overlap extension (SOE) method. The resulting nucleotide sequence was cloned in the pET23a(+) ex‌pression vector under the control of strong bacteriophage T7 transcription and translation signals. The construct obtained was Transferred into the E. coli strain, BL21(DE3) pLysS and IPTG was used for induction of GM-CSF gene and production of the target protein. one mg of protein per liter of cell culture, was obtained as revealed by ELISA.
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نویسندگان مقاله مانا علومی | mana oloomi


سعید بوذری | saeid bouzari


veladimir o ریچنسکی | veladimir o rechinsky


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-455&slc_lang=en&sid=en
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