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Iranian Biomedical Journal، جلد ۱۱، شماره ۴، صفحات ۲۵۹-۲۶۴
عنوان فارسی سطح نیتریک اکساید در سمینال پلاسما و ارتباط آن با آسیبهای DNA اسپرم
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Nitric Oxide Level in Seminal Plasma and Its Relation with Sperm DNA Damages
چکیده انگلیسی مقاله Evidence supports the involvement of nitric oxide (NO) in a Varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, Low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established that high amounts of NO have detrimental effects on DNA. The integrity of sperm DNA is an important factor in successful fertility and embryo development. It is hypothesized that supra physiological concentrations of NO in seminal plasma cause sperm DNA damage. The aim of this study was to determine sperm DNA damage by comet assay and its correlation with NO level in seminal plasma of fertile and infertile men. Methods: Semen samples were collected from 45 patients and 70 healthy donors. The stable metabolites of NO (nitrite and nitrate) in seminal plasma were measured by Griess assay and DNA damage was determined using single cell gel electrophoresis (comet) assay method. Results: The NO concentration in the seminal plasma of infertile males was significantly higher than fertile males (5.74 ± 1.01 &muM/L vs. 3.88 ± 0.53 &muM/L). There was a significant positive correlation between the NO concentration and sperm DNA comet value in infertile males (P< 0.01, R = 0.598). Conclusion: These results indicate that the overproduction of NO in genital tract of infertile males has a potential pathogenetic role in the reduction of sperm DNA integrity.
کلیدواژه‌های انگلیسی مقاله Nitric oxide (NO), Infertility, Sperm, DNA integrity
نویسندگان مقاله ایرج امیری | iraj amiri


نسرین شیخ | nasrin sheikh


رضوان نجفی | rezvan najafi


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-104&slc_lang=en&sid=en
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زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
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