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Avicenna Journal of Phytomedicine، جلد ۸، شماره ۳، صفحات ۲۳۷-۲۴۵
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عنوان انگلیسی Cuscuta campestris induces apoptosis by increasing reactive oxygen species generation in human leukemic cells
چکیده انگلیسی مقاله Objective: Cuscuta campestris or common dodder is a holoparasitic  plant  that  has  been valorized for treatment of liver injury and cancer prevention in traditional medicine. Recently, extract of C. campestris had shown moderate antimicrobial properties and cytotoxic effects. In this study, we examined the level of cellular oxidants, cytotoxicity, apoptosis and differentiation induced by hydroalcoholic extract of C. campestris(CCE)(12.5-200 µg/ml), as well as arsenic trioxide (As2O3, 50 µM), in human leukemic (HL60 and NB4) and normal polymorph nuclear cells after 72 hr treatment. Materials and Methods: Resazurin assay was used to determine cell viability following treatment with C. campestris. Intracellular reactive oxygen species (ROS) and apoptotic cells were measured by fluorimetry using carboxy 2′, 7′-dichlorofluorescein diacetate and propidium iodide (PI), as staining reagents, respectively. The differentiation of leukemic cells was evaluated by Giemsa staining and nitro blue tetrazolium (NBT) reduction. Results: C. campestris inhibited cell viability with IC50 values of 23.9 µg/ml for HL60 and 60.3 µg/ml for NB4 cells after 72 hr treatment. ROS formation was also concentration-dependently increased following treatment with C. campestris. In addition, the number of apoptotic cells significantly increased to 88.4% and 62.3% in CCE (200 µg/ml)-treated HL60 and NB4 cells, respectively, which was higher than that of As2O3 (50 µM)-treated leukemic cells (p< 0.001). Nonetheless, C. campestris did not induce differentiation of leukemic cells towards granulocytic pattern. Conclusion: The present study demonstrated that C. campestris induced apoptosis through ROS production without having differential effect on leukemic cells, in concentration- and time-dependent manners. Understanding of precise signaling pathway by which C. campestris induce apoptosis, needs further research
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نویسندگان مقاله | Maliheh Moradzadeh
Golestan Rheumatology Research Center, Golestan University of Medical Sciences, Gorgan, Iran


| Azar Hosseini
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran


| Hasan Rakhshandeh
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran


| Azita Aghaee
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran


| Hamidreza Sadeghnia
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
نشانی اینترنتی http://ajp.mums.ac.ir/article_10011.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/232/article-232-585121.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Research Article
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