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Caspian Journal of Enviromental Sciences، جلد ۱۵، شماره ۴، صفحات ۳۰۹-۳۱۹
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عنوان انگلیسی Analysis of vitellogenin gene structure in Caspian roach, Rutilus caspicus (Pisces: Cyprinidae) during exposure to Atrazine
چکیده انگلیسی مقاله Chemical contamination of aquatic environments to EDCs has become a major focus of environmental toxicology research. The exposure of fishes to estrogenic EDCs in aquatic environments is most frequently assessed by analyzing Vitellogenin (Vg) (the egg yolk precursor protein) expression. Therefore, characterization of Vg gene is of high priority for EDCs bio-monitoring. So, we prepared liver tissue samples of Caspian roach, Rutilus caspicus for RNA extraction. Following the cDNA synthesis, specifically - designed primers were employed to amplify the Vg gene and ultimately sequence it. The evolutionary analyses of the sequence were performed using MEGA7 software. The obtained results indicated that the designed primers successfully amplified the partial cDNA sequence. Our results indicated that this sequence most probably belongs to the Vg1 form of the gene. Moreover, it was demonstrated that Caspian roach and Petroleuciscus esfahani share a common ancestor. Noteworthy, the study of Vg gene would be helpful to understand the molecular mechanisms of development and would be used to establish a bio-monitoring tool for detection of exposure to different EDCs.
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نویسندگان مقاله m اویسی |
islamic azad university of tehran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

سید حسین جمیلی | s h
agricultural research, education and extension organization tehran

m بهدانی |
biotechnology research center pasteur institue of iran

a ماشینچیان مرادی | mashinchian moradi
islamic azad university of tehran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

e شریف پور |
agricultural research education and extension organization tehran

نشانی اینترنتی http://cjes.guilan.ac.ir/article_2638_db5f241e8aaf9a459909de483a27f6d3.pdf
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زبان مقاله منتشر شده en
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