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Iranian Journal of Parasitology، جلد ۱۲، شماره ۴، صفحات ۶۱۴-۶۲۱
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عنوان انگلیسی Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran
چکیده انگلیسی مقاله Abstract Background: Cystic Echinococcosis (CE) is one of the most common parasitic zoonosis caused by Echinococcus granulosus worldwide. This study investigated genotype diversity of Echinococcus granulosus isolated from stray dogs and golden jackals in Ilam province, West of Iran. Methods: Adult worms were collected from the small intestine of the stray dogs and golden jackals from Ilam Province roads during 2012-2014. DNA was extracted from the adult worms and the partial mitochondrial NADH dehydrogenase subunit1 ( nad1 ) was amplified by PCR then the products were digested by using HpaII , Rsa 1, and Alu 1 restriction enzymes. In order to confirm RFLP results, a number of PCR products were bi-directionally sequenced. Results: Totally, 20 stray dogs out of 75 (26.66%) and two out of 73 (2.74%) golden jackal showed infection with E. granulosus . Amplified PCR product for all isolates was a band of approximately 550bp. Alu 1 digested the product into two bands of approximately 160bp and 390bp fragments, while the Rsa1 cut the product into 320bp and 230bp fragments and the Hpa II had no effect on the PCR product for both dog and jackal samples. The isolate sequences of mtDNA nad1 gene indicated 100% homology with references G1, G2 and G3 sequences in the GenBank database. Conclusion: The genotype of adult E. granulosus was similar to larval stage genotypes of parasite (G1-G3 complex).
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نویسندگان مقاله عبدالحسین دلیمی | abdolhossein dalimi
dept. of parasitology, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

مرتضی شمسی | morteza shamsi
dept. of immunology, school of medicine, ilam university of medical sciences, ilam, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

افرا خسروی | afra khosravi
dept. of parasitology, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

فاطمه غفاری فر | fatemeh ghaffarifar


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1936
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کد مقاله (doi)
زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Short Communication(s)
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