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Iranian Journal of Parasitology، جلد ۱۲، شماره ۴، صفحات ۵۰۶-۵۱۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Diagnostic Accuracy of Loop-mediated Isothermal Amplifica-tion Assay as a Field Molecular Tool for Rapid Mass Screening of Old World Leishmania Infections in Sand Flies and In Vitro Culture
چکیده انگلیسی مقاله Abstract Background: We employed a highly sensitive loop-mediated isothermal amplification (LAMP) by targeting 18S rRNA gene to identify the rapid mass screening of Leishmania infections in captured sand flies of southwest Iran and In vitro culture. Methods: One hundred fifty sand flies were collected from 11 sites adjacent to Iraqi’s borders in southern parts of Khuzestan Province by using sticky sheets of paper and CDC miniature light traps during late May 2014 to Nov 2015. Following morphological identification of sand flies species, the DNA of infected samples was extracted and amplified by PCR and LAMP assays by targeting ITS-rDNA and 18S rRNA genes. The PCR amplicons were directly sequenced to conduct the phylogenetic analysis Results: Ten (6.6%) Leishmania infections were identified by LAMP assay (detection limit 0.01 parasites DNA) among infected Sergentomyia baghdadis , S. sintoni and Phlebotomus papatasi sand flies that was more sensitive than PCR (n=6.4%; (detection limit 10 1 parasites DNA). LAMP can identify 10 1 -10 6 promastigotes/100 µl RPMI 1640 while PCR recognized10 4 -10 6 promastigotes. The majority infection rate of sand flies was confirmed to L. major inferred by phylogenetic analysis. Conclusion: This is the first exploration characterized the Old World Leishmania infections by LAMP technique in both infected sand flies and In vitro conditions. The LAMP method because of its shorter reaction time, robustness, more sensitivity, lack of requirement of complicated equipment and visual discriminatory of positivity can be appeared a promising tool instead of PCR to identify low Leishmania loads and entomological monitoring of leishmaniasis in resource-limited endemic of the world.
کلیدواژه‌های انگلیسی مقاله LAMP, PCR, Leishmania, Sandfly, Iran
نویسندگان مقاله مهدی قدرتی | mehdi ghodrati
immunology research center, tabriz university of medical sciences, tabriz, iran dept. of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

عادل اسپوتین | adel spotin
dept. of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

تیمور حضرتیان | teimour hazratian
dept. of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

محمود محامی اسکویی | mahmoud mahami oskouei
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

علی بردبار | ali bordbar
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

سحر ابراهیمی | sahar ebrahimi
dept. of parasitology and mycology, faculty of medicine, lorestan university of medical sciences, khorramabad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی لرستان (Lorestan university of medical sciences)

شیرزاد فلاحی | shirzad fallahi
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

پرویز پرویزی | parviz parvizi


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1924
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