| چکیده انگلیسی مقاله |
Abstract Background: Rhipicephalus sanguineus is the most widely distributed tick in the world, which is partly due to its biological flexibility and the global distribution of its major host, the domestic dog. In Mediterranean region it could be principal reservoir host for Leishmania infantum, usually transmitted by the phlebotomine sand flies . In this study, we evaluated the vector potential of R. sanguineus in transmitting L. infantum to uninfected dogs. Methods: During 2014, five dogs with clinical manifestations of canine visceral leishmaniasis (CVL), high anti- Leishmania antibody titers and tick infestation, were selected from CVL endemic areas (Tehran and Alborz provinces). At least, twenty live ticks were removed from each infected dog. After morphological identification, the ticks were divided into two groups; ticks belonging to the first group were dissected for parasitological examinations and semi-nested PCR assay, and those of the second group were selected for the transmission of CVL caused by L. infantum to uninfected dogs. Following tick infestation, all uninfected dogs were kept for 9 months and examined monthly for clinical and serological tests. Results: Nearly, 67% of ticks were infected by L. infantum using the semi-nested PCR. All other parasitological tests of ticks were negative. Clinical examinations and serological tests of the investigated dogs revealed negative results. Nested-PCR test results performed on splenic biopsy samples of dogs were also negative. Conclusion: L. infantum -positive R. sanguineus ticks were unable to transfer L. infantum from infected dogs to healthy ones. The detection of L. infantum DNA in ticks collected from naturally infected dogs by semi-nested PCR does not prove their vectorial competence. |
| نویسندگان مقاله |
آلاله رخشانپور | alaleh rakhshanpour
dept. of internal diseases, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
عبدالعلی ملماسی | abdolali malmasi
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
مهدی محبعلی | mehdi mohebali
dept. of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
صدیقه نبیان | sedigheh nabian
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran dept. of medical parasitology and mycology, school of medicine, isfahan university of medical sciences, isfahan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)
حسین میرهندی | hossein mirhendi
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran meshkin-shahr research station, national institute of health research, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
ذبیح اله زارعی | zabihollah zarei
dept. of parasitology, faculty of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
عبدالحسین دلیمی | abdolhossein dalimi
dept. of parasitology, faculty of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
آنیتا محمدیها | anita mohammadiha
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
بهناز آخوندی | behnaz akhoundi
dept. of medical entomology, faculty of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
امراله آذرم | amrollah azarm
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