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Avicenna Journal of Phytomedicine، جلد ۷، شماره ۴، صفحات ۳۷۶-۳۸۸
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چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Curcumin attenuates harmful effects of arsenic on neural stem/progenitor cells
چکیده انگلیسی مقاله Objective: Arsenic, an environmental pollutant, decreases neuronal migration as well as cellular maturation and inhibits the proliferation of neural progenitor cells. Curcumin has been described as an antioxidant and neuroprotective agent with strong therapeutic potential in some neurological disorders. Human adipose-derived stem cells (hADSCs), a source of multipotent stem cells, can self-renew and differentiate into neural cells. The aim of the present study was to investigate the preventive effect of curcumin against arsenic toxic effects on the viability, telomerase activity, and apoptosis of neural stem/progenitor cells (NSPCs) derived from hADSCs. Materials and Methods: The characteristics of human adipose tissue were identified by immunocytochemistry for surface markers namely, CD105, CD73, and CD90. Using neurosphere assay, hADSCs were differentiated into neuronal cells. To characterize neural cells, expression of nestin, SOX2, MAP2, and GFAP were assessed by immunocytochemistry. Cytotoxicity and viability of NSPCs were evaluated by MTT assay. Reactive oxygen species (ROS) generated by arsenic exposure, were measured and caspase 3/7 activity and caspase-3 processing as well as the telomerase activity were determined. Results: The isolated hADSCs positively expressed CD105, CD73, and CD90. Nestin, Sox2, GFAP, and MAP2 were expressed in the neurospheres derived from hADSCs. Curcumin/arsenic co-treatment significantly increased telomerase activity of NSPCs compared to arsenic group. Furthermore, curcumin significantly reduced arsenic-induced apoptosis (via inactivation of caspases) as well as arsenic-associated ROS generation. Conclusion: Our findings revealed that curcumin has the potential to prevent harmful effects of arsenic on neurogenesis.
کلیدواژه‌های انگلیسی مقاله Medicinal plants, Toxicity, Stem cells, Neuronal injury
نویسندگان مقاله علی جهانبازی جهان آباد | ali jahanbazi jahan abad
shefa neuroscience research center, khatam alanbia hospital, tehran, iran

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

پرستو مرتضی زاده | parastoo morteza zadeh
department of biology, kharazmi university of tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

سجاد سحاب نگاه | sajad sahab negah
department of neuroscience, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

علی گرجی | ali gorji
epilepsy research center, department of neurology, and department of neurosurgery, westfälische wilhelms-universität münster, münster, germany
نشانی اینترنتی http://ajp.mums.ac.ir/article_8305.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Research Article
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