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Biomacromolecular Journal، جلد ۲، شماره ۲، صفحات ۱۴۳-۱۵۱
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Assessing Stability of Gold Nanoparticles in Presence of Two Enzymes, RNaseA and RNaseH, Using Colorimetric Detection |
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| چکیده انگلیسی مقاله |
Gold nanoparticle-based diagnostic methods have attracted much attention due to their simplicity, high sensitivity and low cost. These methods are mostly used for early and efficient detection of various pathogenic factors and assessment of gene expression and nanoparticles stability. In this study, melon plants were cultured in vivo and then, RNA was extracted from leaf explants. A 40 bp probe was designed based on the Actin gene sequence and applied to investigate the gene expression by colorimetric detection. Color variation of the nanoparticles, from red to blue was observed in presence of the target molecules. The maximum observed changes was at 550-650 nm. Moreover, the activity and the effect of two enzymes, RNaseA and RNaseH, on the stability of gold nanoparticles were investigated. The results showed the effect of the RNaseA enzyme in the initial time period along with the RNaseH enzyme over the time on the stability of gold nanoparticles. Such colorimetric techniques, which are based on the stability of the gold nanoparticles could be used for rapid assessment and recognition of gene expression and the factors affecting the stability of the gold nanoparticles. These could also be considered as a useful technique for screening other enzymes and medicinal molecules. |
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| نویسندگان مقاله |
یاسر قاضی |
department of biology, faculty of basic sciences, university of zabol, zabol, iran
سازمان اصلی تایید شده: دانشگاه زابل (Zabol university)
فاطمه حدادی |
department of biology, faculty of basic sciences, university of zabol, zabol, iran
سازمان اصلی تایید شده: دانشگاه زابل (Zabol university)
حسین کمال الدینی |
department of biology, faculty of basic sciences, university of zabol, zabol, iran
سازمان اصلی تایید شده: دانشگاه زابل (Zabol university)
سپیده احمدی |
department of biology, faculty of basic sciences, university of zabol, zabol, iran
سازمان اصلی تایید شده: دانشگاه زابل (Zabol university)
اکبر واثقی |
young researchers and elite club, ardabil branch, islamic azad university, ardabil, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://www.bmmj.org/article_25602_ae106e7ebecd241fafc405bfde60d720.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2124/article-2124-465678.pdf |
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| زبان مقاله منتشر شده |
en |
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