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Journal of Medical Bacteriology، جلد ۵، شماره ۳-۴، صفحات ۲۲-۲۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی A Rapid and Reproducible Genomic DNA Extraction Protocol for Sequence-Based Identification of Archaea, Bacteria, Cyanobacteria, Diatoms, Fungi, and Green Algae
چکیده انگلیسی مقاله Background : Sequence-based identification of various microorganisms including Archaea, Bacteria, Cyanobacteria, Diatoms, Fungi, and green algae necessitates an efficient and reproducible genome extraction procedure though which a pure template DNA is yielded and it can be used in polymerase chain reactions (PCR). Considering the fact that DNA extraction from these microorganisms is time consuming and laborious, we developed and standardized a safe, rapid and inexpensive miniprep protocol. Methods : According to our results, amplification of various genomic regions including SSU, LSU, ITS, β-tubulin, actin, RPB2, and EF-1 resulted in a reproducible and efficient DNA extraction from a wide range of microorganisms yielding adequate pure genomic material for reproducible PCR-amplifications. Results : This method relies on a temporary shock of increased concentrations of detergent which can be applied concomitant with multiple freeze-thaws to yield sufficient amount of DNA for PCR amplification of multiple or single fragments(s) of the genome. As an advantage, the recipe seems very flexible, thus, various optional steps can be included depending on the samples used. Conclusion : Having the needed flexibility in each step, this protocol is applicable on a very wide range of samples. Hence, various steps can be included depending on the desired quantity and quality.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله فرخنده صبا | farkhondeh saba
microorganisms bank, iranian biological resource center ibrc , acecr , tehran, iran.


مسلم پاپی زاده | moslem papizadeh
microorganisms bank, iranian biological resource center ibrc , acecr , tehran, iran.


جواد khansha | javad khansha
microorganisms bank, iranian biological resource center ibrc , acecr , tehran, iran.


مهشید صدقی | mahshid sedghi
microorganisms bank, iranian biological resource center ibrc , acecr , tehran, iran.


مهرنوش رسولی | mehrnoosh rasooli
2 extremophiles laboratory, department of microbiology, faculty of biology and center of excellence in phylogeny of living organisms, college of science, university of tehran, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

محمد علی آموزگار | mohammad ali amoozegar
national laboratory of industrial microbiology, department of microbiology, faculty of biological sciences, alzahra university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه الزهرا (Alzahra university)

محمدرضا صعودی | mohammad reza soudi
department of molecular and cellular biology, faculty of basic sciences and advanced technologies in biology, university of science and culture, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علم و فرهنگ (University of science and culture)

سید ابوالحسن شاهزاده فاضلی | seyed abolhassan shahzadeh fazeli


نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/252
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/313/article-313-442057.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
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