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Iranian Journal of Public Health، جلد ۳۶، شماره ۴، صفحات ۹۱-۹۵
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عنوان انگلیسی Tween as a Substitute for Diethyl Ether in the Formalin-Ether Sedimentation Technique
چکیده انگلیسی مقاله Background: Tween as a substitute for diethyl ether in the formalin-ether sedimentation technique was evaluated for para­site detection. Methods: Fresh fecal material free of parasites with 10% formalin to prepare standardized specimen was thoroughly pooled. This specimen was divided into 5 equal portions; one was without infection, and each of the others was individually seeded with Entamoeba coli , and Giardia lamblia cysts, ova of Ascaris lumbericoides , and Hymenolepis nana. Six hundred and eighty four slides including 228 stool samples for each of formalin-tween, formalin-ether and direct wet mount proce­dures were examined. Results: The sensitivity of above mentioned procedures were computed 72.1%, 55% and 30 %; their negative predictive value were 69.3%, 58.3% and 47.3%; and their false negative error rate were 27.9%, 45% and 70%, respectively. There were no false-positive results among the 264 specimens previously identified as negative for the presence of intestinal para­sites. Therefore, specificity for each technique was 100%. Conclusion: In the range of our study, formalin-tween method proved to be equivalent to or better than formalin-ether tech­nique in concentrating parasite eggs, and cysts, as well as in maintaining characteristic morphology. Tween is more stable, safer, cheaper, and less flammable than that of ether; and promises to be a useful alternative to ether.
کلیدواژه‌های انگلیسی مقاله Formalin, Tween
نویسندگان مقاله na احمدی | na ahmadi


k pakdad | k pakdad


نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/2097
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