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Iranian Journal of Public Health، جلد ۳۸، شماره ۱، صفحات ۶۰-۶۸
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عنوان انگلیسی Molecular Detection of Common Bacterial Pathogens Causing Meningitis
چکیده انگلیسی مقاله Background: The clinical diagnosis of meningitis is crucial, particularly in children. The early diagnosis and empiric an­tibi­otic treatments have led to a reduction in morbidity and mortality rates. PCR and the enzymatic digestion of 16SrDNA frag­ment which is produced by universal primers led up fast and sensitive determination. The purpose of this study was to investi­gate a rapid method for detection of common bacterial pathogens causing meningitis. Methods: According to the gene encoding 16SrDNA found in all bacteria, a pair of primers was designed. Then the univer­sal PCR was performed for bacterial agents of meningitis ( Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influ­enzae, etc.) by employing broad- range DNA extraction method. The ob­tained uni­versal PCR products were digested with restriction enzymes ( Hae III, Alu I and Mnl I) to identify bacterial species. Results: By the enzymatic digestion of the universal products of each standard strain of the above bacteria, specific patterns were achieved. These specific patterns may be used for comparison in CSF examination. The analytical sensitivity of the as­say was approximately 1.5´10 2 CFU/ml of CSF even in samples with high amount of proteins. Conclusion: The universal PCR coupled with enzymatic digestion can be used to detect and identify bacterial pathogens in clini­cal specimens rapidly and accurately. Molecular diagnostic of bacterial meningitis, though expensive and labor-inten­sive, but is valuable and critical in patient management.
کلیدواژه‌های انگلیسی مقاله Bacterial diagnosis, Universal PCR, Meningitis
نویسندگان مقاله h صدیقیان | h sadighian
dept. of pathobiology, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

mr پورمند | mr pourmand
pediatrics infections research center, shahid beheshti university m.c , tehran iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/3212
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