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Iranian Journal of Public Health، جلد ۳۸، شماره ۲، صفحات ۱۰۶-۱۱۲
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عنوان انگلیسی Molecular Characterization of Cyclophilin Protein Gene in Skin Normal Microflora: Malassezia furfur
چکیده انگلیسی مقاله Background: Malassezia are dimorphic, lipid-dependent yeasts, which are responsible for causing several cutaneous and sys­temic conditions. Although cyclophilins (CyPs) are highly conserved cytosolic proteins that catalyze the peptidyl-prolyl cis-trans isomerazation reaction before protein folding process, it has been suggestive of an allergen in a few numbers of fungi such as Aspergillus fumigatus and Malassezia species. Allergenic cyclophilins are IgE-binding components, which have been characterized in other species of Malassezia ; and are considered as Mala s 6 in Malassezia sympodialis . In the pre­sent study we tried to identify the molecular characterization of cyclophilin gene in M. furfur. Methods: Pairs of oligonucleotide primers were designed from highly conserved regions of the gene counterparts in other fungi. The primers were then applied to amplify the primer-specific DNA fragment. Afterward, PCR product fragments were sequenced to be used in further analysis. Results: About 573 nucleotides, encoding a polypeptide of 190 amino acids, have been sequenced. Sequence comparison was performed in Gene Bank, both for the nucleotides and their deduced amino acid sequence. It revealed a significant homol­ogy with cyclophilin genes and proteins of other eukaryotic cells. The amino acid sequence of the encoded protein was about 86% identical to the sequence of cyclophilin protein from other fungi. Conclusion: The molecular characterization of cyclophilin gene may open the way to disclosure of the functional char­acteris­tics of cyclophilin and is a fundamental step for understanding the molecular basis of its pathogenesis in AEDS dis­ease.
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نویسندگان مقاله m موذنی | m moazeni
division of molecular biology, dept. of medical mycology amp;amp; parasitology, school of public health, te


f نوربخش | f noorbakhsh
islamic azad university, varamin-pishva branch, tehran, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

زینی | f zaini
division of molecular biology, dept. of medical mycology amp;amp; parasitology, school of public health, te


پریوش کردبچه | p kordbacheh
division of molecular biology, dept. of medical mycology amp;amp; parasitology, school of public health, te


هاشمی | j hashemi
division of molecular biology, dept. of medical mycology amp;amp; parasitology, school of public health, te


b موسوی | b mousavi
division of molecular biology, dept. of medical mycology amp;amp; parasitology, school of public health, te


l آندونیان | l andonian


رضایی | s rezaie
division of molecular biology, dept. of medical mycology amp;amp; parasitology, school of public health, te
نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/3195
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