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Iranian Journal of Public Health، جلد ۳۸، شماره ۳، صفحات ۱۷-۲۳
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عنوان انگلیسی Deletion and Testicular Expression of DAZ (Deleted in Azoospermia) Gene in Patients with Non-Obstructive Azoospermia
چکیده انگلیسی مقاله Background: Deletions of the DAZ (deleted in azoospermia) genes within the human Y chromosome's AZFc region are the most common cause of spermatogenesis failure. These deletions are usually assessed by analyses of genomic DNA ex­tracted from peripheral leukocytes. DAZ genes are expressed in male germ cells. In this prospective study, we investigated DAZ expression and deletion in 102 consecutive infertile men presenting with non-obstructive azoospermia in Avesina Re­search Institute, Tehran, Iran during 2005-6. Methods: In this prospective study, we extracted genomic DNA from peripheral blood leukocytes for detection of DAZ dele­tions and testicular biopsies for histopathological assessment and analyses of DAZ expression level by reverse transcrip­tion polymerase chain reaction. DAZ levels were normalized to expression of the housekeeping Phosphoglu-comu­tase 1 gene . Results: In four out of 102 patients (3.9%), we found DAZ deletion. DAZ expression was observed in 60 (61.2%) of 98 other patients. Expression was not detected in patients with Sertoli cell-only syndrome, but observed in 37 of 40 (92.5%) pa­tients with maturation arrest and 20 of 26 (76.9%) with hypospermatogenesis. Conclusion: The absence of DAZ expression could result in quantitative reduction of germ cells and might be observed de­spite of normal genomic DNA constitution. We recommend to check DAZ testicular expression and genomic DNA deletion, in non-obstructive azoospermia. This is more recommended to avoid transmission of genetic abnormalities which might lead to infertility in male offspring, when assisted reproductive techniques (ART) are performed.
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نویسندگان مقاله m اعرابی | m aarabi
dept. of reproductive genetics and biotechnology, reproductive biotechnology research center, avesin


k سلیمی نژاد | k saliminejad
dept. of reproductive genetics and biotechnology, reproductive biotechnology research center, avesin


mr صادقی | mr sadeghi
dept. of reproductive genetics and biotechnology, reproductive biotechnology research center, avesin


h سلطان قرایی | h soltanghoraee
avesina infertility clinic, avesina research institute, tehran, iran


n امیرجنتی | n amirjannati
avesina infertility clinic, avesina research institute, tehran, iran


mh مدرسی | mh modarressi


نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/3166
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