| چکیده انگلیسی مقاله |
Background and purpose: There is an increasing rate of drug resistance against trichomoniasis and current drugs used in treatment of this disease have many side effects, therefore, attempts have been made to explore alternative drugs favorably from natural sources. The present study aimed at evaluating the anti-Trichomonas effect of essential oil and that of nano-emulsion of Rosmarinus officinalis on the growth of Trichomonas vaginalis (T. vaginalis) in vitro. Materials and methods: T. vaginalis reference strains were cultured in modified TYM medium. The components of rosemary essential oil were determined by gas chromatography. In addition, cytotoxicity effects of essential oils and nano-emulsions prepared from this plant were evaluated against macrophage cell line (J774.A.1). Serial concentrations of 10, 25, 50, and 100 (μg/mL) were prepared from rosemary essential oil and nano-emulsion. Then growth inhibition rate of the parasites in different concentrations was done in triplicate within 1, 2, and 3 hours. Results: Rosemary essential oil and nano-emulsion had an inhibitory effect on the growth of Trichomonas and concentration of 100 μg/mL showed growth inhibition effect of 96.1% and 100% throughout 1,2 and 3 hours, respectively. Additionally, cytotoxicity assay against macrophage revealed no toxicity on the cells at 100 μg/mL. Conclusion: According to the suitable anti-trichomonas activity of rosemary, this plant could be considered as a good candidate in treatment of Trichomonas infection, after complementary studies on animal model. |
| نویسندگان مقاله |
هاجر ضیایی هزار جریبی | hajar ziaei hezar jaribi
associate professor , department of parasitology, molecular and cell biology research center, mazandaran university of medical sciences, sari, iran
دانشیار، گروه انگل شناسی، مرکز تحقیقات بیولوژی سلولی و مولکولی، دانشگاه علوم پزشکی مازندران، ساری، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
نجمه نادعلی | najmeh nadeali
msc student in parasitology, student research committee, faculty of medicine, mazandaran university of medical sciences, sari, iran
دانشجوی کارشناسی ارشد انگل شناسی، کمیته تحقیقات دانشجویی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
مجید سعیدی | majid saeidi
professor, department of pharmaceutics, pharmaceutical sciences research center, faculty of pharmacy, mazandaran university of medical sciences, sari, iran
استاد، گروه فارماکوسیوتیکس، مرکز تحقیقات علوم دارویی، دانشگاه علوم پزشکی مازندران، ساری، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
زهره مومنی | zohreh momeni
assistant professor, department of microbiology, faculty of sciences, islamic azad university, karaj branch, karaj, iran
استادیار، گروه میکروبیولوژی، دانشکده علوم، دانشگاه آزاد اسلامی واحد کرج، کرج، ایران
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی کرج (Islamic azad university of karaj)
مسعود سوسرایی | masoud soosaraei
phd student in parasitology, student research committee, faculty of medicine, mazandaran university of medical sciences, sari, iran
دانشجوی دکتری انگل شناسی، کمیته تحقیقات دانشجویی، دانشکده پزشکی، دانشگاه علوم پزشکی مازندران، ساری، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
مهدی فخار | mahdi fakhar
associate professor , department of parasitology, molecular and cell biology research center, mazandaran university of medical sciences, sari, iran
ساری کیلومتر 18 جاده خزر آباد، مجتمع دانشگاهی پیامبر اعظم ، دانشکده پزشکی
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
اغول نیاز جرجانی | oghol niaz jorjani
assistant professor, department of parasitology, laboratory science research center, faculty of paramedical, golestan university of medical sciences, gorgan, iran
استادیار، گروه انگل شناسی پزشکی، مرکز تحقیقات علوم آزمایشگاهی دانشگاه علوم پزشکی گلستان، گرگان، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)
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