| چکیده انگلیسی مقاله |
Abstract Background: Leishmaniasis as an emerging and reemerging disease is increasing worldwide with high prevalence and new incidence in recent years. For epidemiological investigation and accurate identification of Leishmania species, three nuclear and mitochondrial genes (ITS-rDNA, Hsp70, and Cyt b ) were employed and analyzed from clinical samples in three important Zoonotic Cutaneous Leishmaniasis (ZCL) foci of Iran. Methods: In this cross-sectional/descriptive study conducted in 2014-15, serous smears of lesions were directly prepared from suspected patients of ZCL in Turkmen in northeast, Abarkouh in center and Shush district in southwest of Iran. They were directly prepared from suspected patients and DNA was extracted. Two nuclear genes of ITS-rDNA, Hsp70 and one mitochondrial gene of Cyt b within Leishmania parasites were amplified. RFLP was performed on PCR-positive samples. PCR products were sequenced, aligned and edited with sequencher 4.1.4 and phylogenic analyses performed using MEGA 5.05 software. Results: Overall, 203 out of 360 clinical samples from suspected patients were Leishmania positive using routine laboratory methods and 231 samples were positive by molecular techniques. L. major L. tropica , and L. turanica were firmly identified by employing different molecular genes and phylogenic analyses. Conclusion: By combining different molecular genes, Leishmania parasites were identified accurately. The sensitivity and specificity three genes were evaluated and had more advantages to compare routine laboratory methods. ITS-rDNA gene is more appropriate for firm identification of Leishmania species. |
| نویسندگان مقاله |
احمدرضا اسماعیلی رستاقی | ahmad reza esmaeili rastaghi
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran dept. of medical parasitology amp; mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)
عادل اسپوتین | adel spotin
dept. of biology, tonekabon branch, islamic azad university, tonekabon, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
محمدرضا خاتمی نژاد | mohammad reza khataminezhad
dept. of biology, tonekabon branch, islamic azad university, tonekabon, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
مصطفی جعفرپور | mostafa jafarpour
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
الناز علایی نوین | elnaz alaeenovin
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
نرمین نجف زاده | narmin najafzadeh
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran dept. of biology, tonekabon branch, islamic azad university, tonekabon, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
ندا سمیعی | neda samei
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran dept. of biology, tonekabon branch, islamic azad university, tonekabon, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
ندا طالشی | neda taleshi
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
سمیه محمدی | somayeh mohammadi
molecular systematics laboratory, parasitology department, pasteur institute of iran, tehran, iran
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
پرویز پرویزی | parviz parvizi
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