این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Public Health، جلد ۴۶، شماره ۱۰، صفحات ۱۳۷۹-۱۳۸۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients
چکیده انگلیسی مقاله Abstract Background: Atherosclerosis is a disease that affects large and medium size arteries in the body that underlies coronary heart disease. Several nucleotide changes in mitochondrial tRNA genes have been reported in various diseases. The purpose of the study was to identify hotspot mitochondrial tRNA mutations in atherosclerotic patients. Methods: In this case-control study, the variations of ten mitochondrial tRNA genes (about 50%) were investigated in 70 patients from October 2013 and June 2015 suffered from atherosclerosis. The related mitochondrial area was amplified using PCR methid. The mutation analysis was performed by Single Strand Conformational Polymorphism (SSCP) and Restriction Fragment Length Polymorphism (RFLP). All the positive samples were sequenced. Results: We found one novel heteroplasmic mutation (m.5725T>G) and three reported single nucleotide polymorphisms (SNPs) previously in other diseases including m.5568A>G, m.5711A>G and m.12308A>G. Conclusion: These tRNA mutations can alter their steady state level and affect the structure of tRNA. The role of mitochondrial tRNA mutations in the pathogenesis of atherosclerosis could potentially be important for the understanding of mitochondrial dysfunction in coronary atherosclerotic plaque formation.
کلیدواژه‌های انگلیسی مقاله Atherosclerosis, Mitochondrial tRNA, Mutation, PCR-SSCP
نویسندگان مقاله محمد مهدی حیدری | mohammad mehdi heidari
dept. of biology, faculty of science, yazd university, yazd, iran

سازمان اصلی تایید شده: دانشگاه یزد (Yazd university)

محبوبه درخشانی | mahboobe derakhshani
dept. of biology, faculty of science, yazd university, yazd, iran

سازمان اصلی تایید شده: دانشگاه یزد (Yazd university)

فاطمه صدیقی | fatemeh sedighi
dept. of cardiac surgery, afshar hospital, shahid sadoughi university of medical sciences, yazd, iran


سیدخلیل فروزان نیا | seyed khalil foruzan nia


نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/11249
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/86/article-86-430419.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article(s)
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات