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Journal of Medical Bacteriology، جلد ۶، شماره ۱-۲، صفحات ۱۴-۲۰
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عنوان انگلیسی Detection of tstH Gene in Staphylococcus aureus Isolates from Hospitalized Burnt Children
چکیده انگلیسی مقاله Background : The main cause of toxic shock is TSST-1 toxin which is produced by S. aureus . Finding of TSST-1 toxin in burnt children is very important to prevent TSS and its consequences. Methods : The aim of this study was to investigate the presence of gene encoding TSST-1 toxin in wound specimens by PCR. In this case-control study, 90 children who were admitted to the burn unit, were divided in two groups of 45 patients, namely febrile (cases group) and non-febrile (control group). Samplings were done from the burn wounds and were tested by PCR with specific primers of tstH gene. Finally, all data including demographic characteristics, percentage of burnt surface severity and the PCR results were analyzed, statistically. Results : The positive PCR results indicated the expression of tstH gene in 37.7% of the febrile children and 11.1% of the non-febrile children with a statistically significant difference (p < 0.003). The means and the standard deviations for the percentage of burnt surfaces (i.e. severity) in the samples with the positive and negative PCR results were 30.9±16.93 and 20.09±11.02, respectively with a statistically significant difference (p < 0.01). No difference with respect to age and sex could be detected between positive and negative PCR results. Conclusion : A direct association between the expression of tstH and the occurrence of fever in the burnt children was observed. Furthermore, increased surface area of the wounds was also positively related to the expression of tstH .
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نویسندگان مقاله علی بادامچی | ali badamchi
children amp;amp; 039;s medical center hospital, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

شیما جوادی نیا | shima javadinia
imam khomeini hospital, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

بهنام ثبوتی | behnam soboti
department of pediatric infectious disease, research center of pediatric infectious diseases, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

آذردخت طباطبایی | azardokht tabatabaie
department of pediatric infectious disease, research center of pediatric infectious diseases, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

نشانی اینترنتی http://jmb.tums.ac.ir/index.php/jmb/article/view/297
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/313/article-313-414667.pdf
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
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