این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۱۲، شماره ۲، صفحات ۲۷۴-۲۸۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The Impact of Different Copro-preservation Conditions on Molecular Detection of Cryptosporidium Species
چکیده انگلیسی مقاله Background: We aimed to evaluate different copro-preservation conditions along the duration of one month for a better outcome of molecular diagnosis of Cryptosporidium species. Methods: Ten samples out of 380 fresh stool samples collected from patients with diarrhea proved positive after direct examination, concentration, staining and confirmed by immunochromatographic test. The study was conducted at the Diagnostic and Research Unit of Parasitic diseases, Faculty of Medicine, Ain Shams University at the time interval from July 2014 to December 2015. Each stool sample was preserved in five different conditions; freezing at -20 ºC, 70% ethyl alcohol, 10% formalin, 2.5% potassium dichromate (K dichromate) at 4 ºC and 2.5% K dichromate at room temperature (RT). Then DNA extraction and nested PCR, with Cryptosporidium oocyst wall protein (COWP) gene were done from each sample at zero time (fresh specimens) as a standard for comparison with the preservation conditions at 10, 20 and 30 d. Results: Sensitivity of studied preservative conditions along the whole study duration showed best outcome from freezing at -20 °C (80%) then K dichromate (4 °C) (73.3%) followed by K dichromate (RT) (66.7%), then alcohol (33.3%), while formalin was the worst (0%) with a highly significant comparative outcome between the different conditions. Along the three extraction intervals, K dichromate (RT), unlike all the rest of conditions lacks the consistent preservative action. Conclusion: Our study highlights freezing at -20 ºC to be the most suitable condition for preservation followed by K dichromate at 4 °C, K dichromate at RT, then 70% ethyl alcohol. Formalin (10%) is better to be avoided.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله ایمان moawad abdelsalam | iman moawad abdelsalam
dept. of medical parasitology, faculty of medicine, ain shams university, cairo, egypt


rania محمد سرحان | rania mohammad sarhan
dept. of medical parasitology, faculty of medicine, ain shams university, cairo, egypt


مرمر احمد hanafy | marmar ahmed hanafy


نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1634
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-403720.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article(s)
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات