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Iranian Journal of Parasitology، جلد ۱۲، شماره ۲، صفحات ۲۶۰-۲۶۶
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عنوان انگلیسی Parasitological, Serological and Molecular Study of Dirofilaria immitis in Domestic Dogs, Southeastern Iran
چکیده انگلیسی مقاله Background: Dirofilariasis is a serious and potentially deadly condition in dogs and one of the zoonotic filarial infections, which inadvertently affects the humans. The objectives of this study were to determine the seroprevalence and the molecular identity of dirofilariasis in Kerman Province, southeastern Iran between Jul and Aug 2013. Methods: A hundred and forty-nine domestic dogs were randomly selected and five ml blood samples were taken from each dog. One ml of anticoagulant (EDTA) was used for each test in the parasitological study (modified Knott´s test) and sera samples were examined, using ELISA kit to detect Dirofilaria immitis antigen. Extracted DNA of all positive blood samples was used for molecular characterization and sequencing. Results: Four (2.7%) domestic dogs of the total 149 domestic dogs were infected with micofilariae of D. immitis , while the serological study showed 8 (5.4%) domestic dogs were infected with D. immitis . No signifi­cant difference, however, was found between dirofilariasis infec­tion and gender. On the other hand, a signifi­cant difference was observed between dirofilariasis infec­tion and age (P < 0.05 ). Based on the PCR findings, among the total specimens, 6 positive samples were characterized as D. immitis. Conclusion: Dirofilariasis occurred when there was low endemicity in the dogs. Such dogs could be a potential source of infection for humans. These findings could help in better understanding of the epidemiological aspects of D. immitis in the southeastern parts of Iran.
کلیدواژه‌های انگلیسی مقاله Dirofilaria immitis, Domestic dog, Diagnostic tests, Iran
نویسندگان مقاله مهدی بامروت | mehdi bamorovat
leishmaniasis research center, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

ایرج شریفی | iraj sharifi
leishmaniasis research center, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

مجید فصیحی هرندی | majid fasihi harandi
research center for hydatid disease in iran, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

سعید نصیبی | saeed nasibi
research center for hydatid disease in iran, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

بلال صادقی | balal sadeghi
dept. of food hygiene and public health, faculty of veterinary medicine, shahid bahonar university of kerman, kerman, iran

سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)

جواد خدری | javad khedri
dept. of pathobiology, faculty of veterinary medicine, shahid bahonar university of kerman, kerman, iran

سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)

محمد علی محمدی | mohammad ali mohammadi
research center for hydatid disease in iran, kerman university of medical sciences, kerman, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1632
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-403718.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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