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International Journal of Hematology-Oncology and Stem Cell Research، جلد ۲، شماره ۳، صفحات ۱-۵
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عنوان انگلیسی Frequency of BCR-ABL Fusion Transcript in Iranian Patients with Chronic Myeloid Leukemia
چکیده انگلیسی مقاله Introduction: Reverse transcriptase-polymerase chain reaction (RT-PCR) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemia cells. A specific chromosomal abnormality, the Philadelphia chromosome (Ph), is present in 90% to 95% of CML patients.The aberration results from a reciprocal translocation between chromosome 9 and 22, creating a BCR-ABL fusion gene.There are two major forms of the BCR/ABL fusion gene, involving ABL exon 2, but including different exons of BCR gene. The transcripts b2a2 or b3a2 code for a p210 protein. Another fusion gene leads to the expression of an e1a2 transcript, which codes for a p190 pro-tein. Another, less common fusion genes are b3a3 or b2a3 (p203) and e19a2 (p230). The incidence of one or other rearrangement in chronic myeloid leukemia (CML) patients varies in different reported se-ries. In general, fusion transcripts are determined individually, a process which is labor intensive in or-der to detect all major fusion transcripts. Methods: This study was designed to determine the frequency of different fusion genes in 75 iranian patients with CML. peripheral blood samples were analyzed by multiplex reverse transcriptase poly-merase chain reaction (RT-PCR) from adult patients to detect all types of BCR-ABL transcripts of the t (9:22) and found that all cases were positive for some type of BCR/ABL rearrangement. Results: Most of our patients showed b3a2 fusion gene (62%), while the remaining showed one of the transcripts of b2a2, b3a3, b2a3, e1a2 or coexpression of b3a2 and b2a2. The rate of coexpression of the b3a2 and b2a2 was 5%. Conclusion: In contrast to the other reports, we did not see any coexpression of p210/p190. This may reflect either the sensitivity of the detection techniques used or the possibility of genetic differences be-tween the populations studied. Coexpression may be due to alternative splicing or to phenotypic varia-tion, with clinical course different from classical CML.
کلیدواژه‌های انگلیسی مقاله BCR-ABL,CML,Multiplex RT-PC
نویسندگان مقاله m یغمایی | m yaghmaie
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran


s h غفاری | s h ghaffari
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran


k علی مقدم | k alimoghaddam
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran


a قوام زاده | a ghavamzadeh
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran


موسوی | s a mousavi
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran


m ایروانی | m irvani
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran


b بهار | b bahar
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran


e bibordi | e bibordi
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran


m جهانی | m jahani
hematology-oncology and bone marrow transplantation research center, shariati hospital,tehran, iran
نشانی اینترنتی http://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/192
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