این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
International Journal of Hematology-Oncology and Stem Cell Research، جلد ۸، شماره ۴، صفحات ۱۲-۱۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Differentiation of bone marrow mesenchymal stem cells into chondrocytes after short term culture in alkaline medium.
چکیده انگلیسی مقاله Background: Bone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body. Methods: MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical factors such as pH could recommend new approaches for preparation of suitable scaffolds for bone and cartilage tissue engineering. In present study, the effect of alkaline medium on chondrogenic and osteogenic differentiation of rat MSCs was evaluated.MSCs were harvested from bone marrow of animals and then the response of passage1 and 2 of MSCs (P1 MSCs & P2 MSCs) to the culture in alkaline medium (pH: 8) was evaluated. Cytochemical and immunocytochemical staining were performed to distinguish chondrocytes and osteocytes. Real-time PCR was performed to evaluate the type II collagen and osteopontin mRNA levels. Results: Staining for type II collagen, a chondrocytic specific marker, revealed that after one-week culture in alkaline medium, a considerable amount of P1 MSCs had shown chondrocytic morphology. By prolonging the culture period up to 4 weeks, osteogenic cells with expanded matrix and mineralized areas around them were appeared. Results of real-time PCR showed that P1 MSCs after one week culture in alkaline medium expressed highest rate of type II collagen and osteopontin mRNA among all groups. Conclusion: This study demonstrated that alkaline medium is a potent chondrogenic differentiation inducer for MSCs in their first passage.
کلیدواژه‌های انگلیسی مقاله Alkaline medium,Chondrocyte,Mesenchymal stem cell,Osteoblast
نویسندگان مقاله فرشاد همایونی مقدم | farshad homayouni moghadam
department of physiology, school of medicine, shahid sadoughi university of medical sciences, yazd, iran ; neurobiomedical research center, school of medicine, shahid sadoughi university of medical sciences, yazd, iran.


طاهره طیبی | tahereh tayebi
department of physiology, school of medicine, shahid sadoughi university of medical sciences, yazd, iran.


مریم دهقان | maryam dehghan
neurobiomedical research center, school of medicine, shahid sadoughi university of medical sciences, yazd, iran.


گیلدا اسلامی | gilda eslami
department of medical parasitology and mycology, school of medicine, shahid sadoughi university of medical sciences, yazd, iran.


حمید ندری | hamid nadri
department of medicinal chemistry, school of pharmacy, shahid sadoughi university of medical sciences, yazd, iran.


علیرضا مرادی | alireza moradi
department of medicinal chemistry, school of pharmacy, shahid sadoughi university of medical sciences, yazd, iran.


حسنعلی واحدیان اردکانی | hassanali vahedian ardakani
department of internal medicine, school of medicine, shahid sadoughi university of medical sciences, yazd, iran.


کاظم برزگر | kazem barzegar
english language department, school of medicine, shahid sadoughi university of medical sciences, yazd, iran.
نشانی اینترنتی http://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/408
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات