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Journal of Medical Microbiology and Infectious Diseases، جلد ۴، شماره ۱، صفحات ۱-۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Serologic Detection of Helicobacter pylori Infection and the Required Cautions
چکیده انگلیسی مقاله Introduction: Helicobacter pylori, as an etiologic cause of peptic ulcers and gastric cancer, should be tested and treated. The true state of infection can only be detected by isolation of gastric biopsies through the invasive method of gastroscopy. However, there are several non-invasive methods for detection of infection, the most common of which is serology. Methods: Here we have evaluated the efficacy of two commonly used commercial IgG-based ELISA kits (Kit-1 and Kit-2) against the endoscopy (biopsy)-based methods of rapid urease test (RUT) and bacterial culture. Our study population included 754 subjects categorized as having: 1) nonulcer dyspepsia (NUD, N=485), 2) peptic ulcer disease (PUD, N=65), and 3) gastric cancer (GC, N=204). Results: The rates of agreement between the results obtained by Kit-1, Kit-2 and both kits with culture/RUT were 62.4% (318 of 500, P=0.0001), 74.4% (183 of 246, P< 0.0001), and 81.8% (117 of 143, P< 0.0001), respectively. The agreement rate between the two kits, regardless of the results of the culture and RUT, was 83.6% (147 of 176, P< 0.0001), leaving 16.4% of the subjects with discrepant results. The sensitivity rate and more drastically the specificity rates (against biopsy-based tests), claimed by Kit-1 (100% and 75%) and Kit-2 (>96% and >99%) were significantly reduced (Kit-1: 94.4% and 40.3%; Kit-2: 86.5% and 71%) for the Iranian population. Conclusion: Our data raises questions regarding the accuracy of commercial IgG-based ELISA kits for the detection of H. pylori infection. Therefore, caution should be practiced when such tests are used as the sole basis of medical decision making.
کلیدواژه‌های انگلیسی مقاله Serology, ELISA, Sensitivity, Specificity, Gold Standard
نویسندگان مقاله مریم اسماعیلی | maryam esmaeili
department of medical biotechnology, biotechnology research center, pasteur institute of iran, tehran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

سمانه صابری | samaneh saberi
department of medical biotechnology, biotechnology research center, pasteur institute of iran, tehran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

مهدی علیخانی | mehdi alikhani
department of medical biotechnology, biotechnology research center, pasteur institute of iran, tehran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

مژگان هاتفی | mojgan hatefi
department of medical biotechnology, biotechnology research center, pasteur institute of iran, tehran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

محمد tashakoripour | mohammad tashakoripour
2department of gastroenterology, amiralam hospital, tehran university of medical sciences, tehran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمود اسحاق حسینی | mahmoud eshagh hosseini
2department of gastroenterology, amiralam hospital, tehran university of medical sciences, tehran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد علی محققی | mohammad ali mohagheghi
cancer research center, tehran university of medical sciences, tehran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مرجان محمدی | marjan mohammadi
department of medical biotechnology, biotechnology research center, pasteur institute of iran, tehran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نشانی اینترنتی http://jommid.pasteur.ac.ir/browse.php?a_code=A-10-52-2&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/350/article-350-399857.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Other
نوع مقاله منتشر شده Original article
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