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Iranian Journal of Parasitology، جلد ۵، شماره ۱، صفحات ۴۱-۴۶
عنوان فارسی
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عنوان انگلیسی Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis
چکیده انگلیسی مقاله Background: Considering that ELISA method presently is the test of choice for diagnosis of fasciolo­sis, the present study was undertaken to evaluate the maximum validity of coated plates at dif­ferent temperatures and different times during one year of evaluation. Methods: Serum samples of patients infected with fasciolosis (n=10), hydatidosis (n=5), toxocaria­sis (n=5), and negative control sera (n=5) were examined. Two series of plates were consid­ered. The first series were coated with Fasciola homogenate Ag 12 ug/ml, and after some steps were blocked with gelatin and preserved at different temperatures as -80 °C , -20 °C, -4 °C and +4°C. The 2 nd series were treated under the same criteria but were not blocked with gelatin. Each series were examined by ELISA test from 1 st month to 12 th month. Sera with 1:125 dilution, and peroxidase-conjugated goat anti-human IgG diluted 1:10000 were considered optimum. Results: To ease reporting the results and due to many similarities only results related to 1 st , 6 th and 12 th months were analyzed and sensitivity, specificity plus cut-off were determined for each series separately. Conclusion: Preserving the coated plates, while unblocked at -80°C for 6-8 months is pertinent and functional and in that case, we can be sure the best out put would be applicable.
کلیدواژه‌های انگلیسی مقاله Fasciolosis,ELISA,Plate,Validity,Sensitivity,Specificity
نویسندگان مقاله mb رکنی | mb rokni
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m aryaeipour | m aryaeipour
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

s کوشا | s koosha
parasitology and mycology division, laboratory sciences department, paramedical school, shaheed beheshti university m.c., tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

mt رحیمی | mt rahimi
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/125
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