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Iranian Journal of Parasitology، جلد ۵، شماره ۳، صفحات ۱-۱۰
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عنوان انگلیسی Serological Evaluation of EgAgB16 kDa, a Recombinant Antigen from Echinococcus granulosus for Diagnosis of Human Hydatidosis
چکیده انگلیسی مقاله Background: Regarding that accurate diagnosis of human hydatidosis still needs more investigations, the present study was conducted to clone, express, and evaluate the gene encoding AgB subunits (EgAgB16 kDa) from Echinococcus granulosus (Iranian G1 strain) and its evaluation by ELISA test. Methods: DNA was extracted from protoscoleces and was utilized by PCR for strain identification. Total RNA was prepared with RNeasy protect mini kit from E. granulosus (Iranian G1 strain) protoscoleces collected from naturally infected sheep with hydatid cyst. Recombinant AgB16 kDa was produced using pETDuet as vector and evaluated by ELISA method. A panel of sera including hydatid cyst-infected individu­als (n=72), healthy individual (n=48), toxoplasmosis (n=4), strongyloidosis (n=4), kala-azar (n=5) and tuberculosis (n=5) were examined using this recombinant antigen. Results: Recombinant protein was purified by affinity chromatography using His-Tag column. After purifica­tion, recombinant protein was confirmed by western blot analysis using His Tag monoclonal anti­body or hydatid positive human serum. The sensitivity, specificity; positive and negative predictive values were calculated as 93.5%, 95.6%, 96% and 92.9%, in that order. The cut-off point was detected 0.3 for rAgB16. Conclusion: While the produced recombinant AgB16 kDa showed promising results in diagnosing human hy­datidosis, but more investigations should be implemented to reach an accurate gold standard.
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نویسندگان مقاله j عبدی | j abdi
department of medical parasitology amp;amp; mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

b کاظمی | b kazemi
department of parasitology, shahid beheshti university, m.c., tehran, iran and cellular and molecular biology research center, shahid beheshti university, m.c., tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

a هانیلو | a haniloo
department of medical parasitology amp;amp; mycology, school of medicine, zanjan university of medical sciences, zanjan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زنجان (Zanjan university of medical sciences)

m محبعلی | m mohebali
department of medical parasitology amp;amp; mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمودی | m mahmoudi
department of epidemiology and biostatistics, medical parasitology amp;amp; mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

رضایی | s rezaei
department of medical parasitology amp;amp; mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

m بنده پور | m bandehpour
cellular and molecular biology research center, shahid beheshti university, m.c., tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

l ماغن | l maghen
cellular and molecular biology research center, shahid beheshti university, m.c., tehran, iran

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

mb رکنی | mb rokni
department of medical parasitology amp;amp; mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/140
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