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Iranian Journal of Parasitology، جلد ۵، شماره ۴، صفحات ۵۵-۶۲
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عنوان انگلیسی Evaluation of Confounders in Toxoplasmosis Indirect Fluorescent Antibody Assay
چکیده انگلیسی مقاله Background : The IFA test is one of the most usual methods for detecting anti- Toxoplasma antibod­ies, although it has not any unique standardization. It seems that the microscopic judg­ment of results is an important confounder in IFA test. Therefore, we conducted the present study to clarify the role of microscopic observer, and other confounders on the test. Methods: Eighty sera were collected from patients suspicious to toxoplasmosis for detection IgG anti- T. gondii by this test. Samples were examined against different series of antigens, IgG anti-human conjugates, and observers. Results: There were no significant differences between the two series of antigens and conjugates. For the observers groups the kappa coefficient of the test results in the experts group (0.97, 0.94-1.00) were significantly higher than the less experienced observers (0.77, 0.68-0.87). C onclusion: We recommend the IFA test to be performed only in reference laboratories and by laboratory technicians that have enough experience for this test. Otherwise, we suggest the substitution of this test with other tests like ELISA for the diagnosis and epidemiological studies.
کلیدواژه‌های انگلیسی مقاله Toxoplasma gondii,IFA,Serology
نویسندگان مقاله m سرایی | m saraei
dept. of medical parasitology and mycology, school of medicine, qazvin university of medical sciences, qazvin, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

s شجاعی | s shojaee
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

ar اسماعیلی | ar esmaeli
dept. of medical parasitology, pasture institute, tehran, iran


h جهانی هاشمی | h jahani hashemi
dept. of biostatistics, school of medicine, qazvin university of medical sciences, qazvin, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی قزوین (Qazvin university of medical sciences)

h کشاورز | h keshavarz
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/158
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