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Iranian Journal of Parasitology، جلد ۵، شماره ۴، صفحات ۲۱-۳۰
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عنوان انگلیسی Molecular and Biomorphometrical Identification of Ovine Babesiosis in Iran
چکیده انگلیسی مقاله Background: Ovine babesiosis is the most important haemoparasitic tick-borne disease of small ruminants in Iran caused by Babesia ovis, B. motasi, and B. crassa . The aim of this study was to characterize the species of ovine Babesia species isolated from different geographical region of Iran. Methods: One hundred fifty four blood samples collected from animals, which demonstrated the pale mucous membranes or hyperthermia. The specimens were transferred to the laboratory and the blood smears stained with Geimsa, the morphological and biometrical data of parasite in any infected erythrocyte have been considered. Extracted DNA from each blood samples were used in PCR and semi nested- PCR in order to confirm the presence of the species. Results: The results of the PCR assays showed nine (5.85%), 81 (53%) and 18 (11.7%) were distinguished as Babesia , Theileria and mixed infection, respectively. Semi nested- PCR did not confirm the presence of B. motasi . Conclusion: The causative organism of many cases of haemoprotozoal diseases, which recorded in previous studies, could be B. ovis or Theileria lestoquardi. The result confirmed that B. ovis was only species which causes babesiosis in the study areas. It seems that the biometrical polymor­phisms could exist in B. ovis in Iran. This polymorphism could be a main problem in differen­tiation between B. ovis and B. motasi and it could be dissolved by specific PCR analysis.
کلیدواژه‌های انگلیسی مقاله Babesia ovis,Babesia mutasi,PCR,Semi nested – PCR
نویسندگان مقاله z صادقی دهکردی | z sadeghi dehkordi
dept. of parasitology, veterinary faculty, university of tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

s ذاکری | s zakeri
malaria and vector research group mvrg , biotechnology research center, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

s نبیان | s nabian
dept. of parasitology, veterinary faculty, university of tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

a باهنر | a bahonar
dept of food hygiene amp;amp; quality control, veterinary faculty, university of tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

f قاسمی | f ghasemi
malaria and vector research group mvrg , biotechnology research center, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

f نوراللهی | f noorollahi
parasitic diseases control group, iranian veterinary organization, tehran, iran


s رهبری | s rahbari
dept. of parasitology, veterinary faculty, university of tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/153
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