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Iranian Journal of Parasitology، جلد ۵، شماره ۴، صفحات ۱-۸
عنوان فارسی
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عنوان انگلیسی Comparison of Three Methods for Diagnosis of Cutaneous Leishmaniasis
چکیده انگلیسی مقاله Background : Leishmaniasis is one of the infectious parasitic diseases of highest incidence in the world. Cutaneous Leishmaniasis (CL) has long been reported in Shiraz, Southern Iran. There is a need to find a sensitive and specific method for treatment and control of the disease. Methods: We have compared the sensitivity of the conventional methods microscopy and cultiva­tion of lesion scrapes against PCR amplification of parasite kinetoplast DNA from these sam­ples. The samples (n=219) were obtained from the patients clinically suspected of CL. The smears were stained with Giemsa for microscopy and cultured in Novy-Nicolle-McNeal (NNN) blood agar for promastigote growth. For PCR, the dry smears were scraped off the slides and DNA was extracted. Results : The positive rates from 219 specimens were 76.71%, 50.68%, and 93.61% for micros­copy, cultivation, and PCR, respectively. The highest correlation was found between PCR and micros­copy method ( P = 0.014). In PCR assay, 95.61%, 3.9%, and 0.49% of the samples were identi­fied as Leishmania major , L. tropica , and dermatropic L. infantum , respectively. Conclusion: The PCR method appears to be the most sensitive for the diagnosis of CL and is valu­able for identifying the other species of Leishmania with confusing dermatropic signs.
کلیدواژه‌های انگلیسی مقاله PCR,Diagnosis,Cultivation,Cutaneous Leishmaniasis
نویسندگان مقاله mh معتضدیان | mh motazedian
dept. of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

gr حاتم | gr hatam
dept. of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

m کلانتری | m kalantari
dept. of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

p حبیبی | p habibi
dept. of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

b سرکاری | b sarkari
dept. of parasitology and mycology, school of medicine, shiraz university of medical sciences, shiraz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/150
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